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Fate-mapping in wild-typeDrosophila melanogaster

II. Injections of horseradish peroxidase in cells of the early gastrula stage
  • Gerhard M. Technau
  • Jose A. Campos-Ortega
Article

Summary

Horseradish peroxidase (HRP) was used as an intracellular marker to follow the fate ofDrosophila embryonic cells. Injections were performed into 6- to 8-min-old gastrulae, when the cells have lost their connections with the yolk sack. Staining for HRP was made at the stage of germ band shortening, when most of the larval structures can be identified. By injecting at approximately 85 different sites we were able to locate the anlagen of the following structures: (a) epidermis: abdominal, thoracic and gnathal segments, dorsal ridge, procephalic lobe, clypeolabrum; (b) gut: pharynx, oesophagus, anterior and posterior midgut, proctodeum, Malpighian tubules and salivary glands; (c) central nervous system: abdominal, thoracic, sub-oesophageal and supra-oesophageal ganglia; (d) amnioserosa. The method proved to be useful in order to analyse morphogenetic movements. The anterior portion of the anterior midgut is shown to be formed from cells which invaginate with the stomodeum; the procephalic field is shown to rotate.

Key words

Fate-mapping Morphogenetic movements HRP Drosophila 

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Gerhard M. Technau
    • 1
  • Jose A. Campos-Ortega
    • 1
  1. 1.Institut für Entwicklungsphysiologie der Universität zu KölnKöln 41Federal Republic of Germany

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