Summary
Supernumerary limbs were produced by deviating the sciatic nerve to the surface of the axolotl hindlimb either alone or in combination with small skin grafts from specific limb positions. With no skin grafts a very low frequency of good supernumeraries were produced. However, when associated with skin grafts, this frequency was significantly increased. The pattern of skeletal elements and muscles were analysed in the supernumeraries which formed at each location. In both the anterior-posterior and dorsal-ventral axes specific anatomical features were found which correlated with their position of origin on the host limb. Characteristic features were also observed with respect to the proximal-distal axis of the outgrowths. These phenomena are discussed in relation to our current understanding of the rules of pattern regulation in the regenerating limb.
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References
Bischler V (1926) L'influence du squelette dans la régénération, et les potentialities des divers territoires du membre chez Triton cristatus. Rev Suisse Zool 33:431–560
Bodemer CW (1958) The development of nerve induced supernumerary limbs in the adult newt,Triturus viridescens. J Morphol 102:555–581
Bodemer CW (1959) Observations on the mechanism of induction of supernumerary limbs in adultTriturus viridescens. J Exp Zool 140:79–101
Bodemer CW (1960) The importance of quantity of nerve fibres in development of nerve-induced limbs inTriturus, and enhancement of the nervous influence by tissue implants. J Morphol 107:47–59
Bryant SV (1978) Pattern regulation and cell commitment in amphibian limbs. In: S Subtelny, I Sussex (eds) The clonal basis of development. 36th symposium of the Society for Developmental Biology. Academic Press, New York, pp 63–82
Bryant SV, Iten LE (1976) Supernumerary limbs in amphibians: experimental production inNotophthalmus viridescens and a new interpretation of their formation. Dev Biol 50:212–234
Bryant SV, French V, Bryant PJ (1982) Distal regeneration and symmetry. Science 212:993–1002
Bryant SV, Holder N, Tank PW (1982) Cell-cell interaction and distal outgrowth in amphibian limbs. Am Zool 22:143–152
DeBoth NJ (1970) The developmental potencies of the regeneration blastema of the axolotl limb. Wilhelm Roux's Arch 165:242–276
Faber J (1960) An experimental analysis of regional organization in the regenerating fore-limb of the axolotl.Ambystoma mexicanum. Arch Biol 71:1–72
French V, Bryant PJ, Bryant SV (1976) Pattern regulation in epimorphic fields. Science 193:969–981
Goss RJ (1957) The relation of skin to defect regulation in regenerating half limbs. J Morphol 100:547–564
Holder N, Tank PW, Bryant SV (1980) Regeneration of symmetrical forelimbs in the axolotl,Ambystoma mexicanum. Dev Biol 74:302–314
Lheureux E (1977) Importance des associations de tissus du membre dans le développment des membres surnuméraires induits par déviation de nerf chez le TritonPleurodeles waltlii Michah. J Embryol Exp Morphol 38:151–173
Maden M (1977) The regeneration of positional information in the amphibian limb. J Theoret Biol 69:735–753
Maden M (1979) Regulation and limb regeneration: the effect of partial irradiation. J Embryol Exp Morphol 52:183–192
Maden M (1980) Structure of supernumerary limbs. Nature 287:803–805
Maden M (1982) Supernumerary limbs in amphibians. Am Zool 22:131–142
Maden M, Mustafa K (1982) The structure of 180 degree supernumerary limbs and a hypothesis of their formation. Dev Biol 93:257–265
Meinhardt H (1983) A bootstrap model for the proximodistal pattern formation in vertebrate limbs. J Embryol Exp Morphol 76:139–146
Papageorgiou S, Holder N (1983) The structure of supernumerary limbs formed after 180 degree blastemal rotation in the newtTriturus cristatus. J Embryol Exp Morphol 74:143–158
Reynolds S, Holder N, Fernandes M (1983) The form and structure of supernumerary hindlimbs formed following skin grafting and nerve deviation in the newt,Triturus cristatus. J Embryol Exp Morphol 77:221–241
Slack JMW (1980) Morphogenetic properties of the skin in axolotl limb regeneration. J Embryol Exp Morphol 58:265–288
Smith AR, Lewis JH, Crawley AM, Wolpert L (1974) A quantitative study of blastemal growth and bone regression during limb regeneration inTriturus cristatus. J Embryol Exp Morphol 32:375–390
Stocum DL (1968) The urodele limb regeneration blastema: a self-organizing system. 11. Morphogenesis and differentiation of autografted whole and fractional blastemas. Dev Biol 18:457–480
Stocum DL (1978) Regeneration of symmetrical hindlimbs in larval salamanders. Science 200:790–793
Stocum DL (1980) Intercalary regeneration of symmetrical thighs in the axolotl,Ambystoma mexicanum. Dev Biol 79:276–295
Tank PW (1978) The occurrence of supernumerary limbs following blastemal transplantation in the regenerating forelimb of the axolotl,Ambystoma mexicanum. Dev Biol 62:143–161
Tank PW (1981) Pattern formation following 180 degree rotation of regeneration blastemas in the axolotl,Ambystoma mexicanum. J Exp Zool 217:377–387
Tank PW, Holder N (1978) The effect of healing time on the proximo-distal organisation of double-half forelimb regenerates in the axolotl,Ambystoma mexicanum. Dev Biol 66:72–85
Tank PW, Holder N (1979) The distribution of cells in the upper forelimb of the axolotl,Ambystoma mexicanum. J Exp Zool 209:435–442
Wolpert L, Tickle C, Sampford M (1979) The effect of cell killing by x-irradiation in pattern formation in the chick limb. J Embryol Exp Morphol 50:175–198
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Maden, M., Holder, N. Axial characteristics of nerve induced supernumerary limbs in the axolotl. Wilhelm Roux' Archiv 193, 394–401 (1984). https://doi.org/10.1007/BF00848230
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DOI: https://doi.org/10.1007/BF00848230