Summary
The exact localization of the presumptive trunk organizer was determined by means of vital staining at the initiation of gastrulation (0 h embryo) and subsequently in 6, 9, 12 and 24 h embryos.
The progressive changes in the self-differentiation and inductive capacity of the trunk organizer were studied in isolation cultures (sitting drop) and in sandwich cultures with competent gastrula ectoderm. In the 0 and 6 h embryo cultures the excised trunk organizer predominantly formed atypical ectoderm. A dramatic change in differentiation and inductive capacity occurred in the 9 h embryo. The positive cases — 83% of the isolation and 50% of the sandwich cultures — mainly formed notochord and somites, accompanied by spinal cord and hindbrain in the sandwich cultures. Although no further change in self-differentiation occurred from that time onwards, a gradual increase in inductive capacity was recognized.
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Kanéda, T., Hama, T. Studies on the formation and state of determination of the trunk organizer in the newt,Cynops pyrrhogaster . Wilhelm Roux' Archiv 187, 25–34 (1979). https://doi.org/10.1007/BF00848164
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DOI: https://doi.org/10.1007/BF00848164