Summary
These experiments examined whether inDrosophila immature imaginal disc tissue and tissues from embryonic stages can influence pattern regulation in a disc fragment in the same way as can mature imaginal discs. Immature imaginal discs, or the cells of whole embryos, were mixed with a test fragment (presumptive notum) from a mature wing disc. The immature tissues in each mixture were genetically marked and had been heavily irradiated (25 Kr gamma) prior to mixing to prevent growth and maturation during subsequent culture in vivo. Alteration of the regulative behavior of the test fragment (that is, regeneration of wing) thus provided an assay for the communication of positional information by the immature tissues. The results suggest that this capacity arises well before competence to metamorphose, as early as the 16th hour of embryonic development, whereas prior to 16 h, essentially no stimulation of regeneration occurred. It is suggested that the imaginal disc (or presumptive disc) cells of the embryo may have been responsible for this early stimulatory capacity.
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References
Adler, P.N., Bryant, P.J.: Participation of lethally irradiated imaginal disc tissue in pattern regulation inDrosophila. Dev. Biol.60, 298–304 (1977)
Bodenstein, D.: Investigations on the problem of metamorphosis. V. Some factors determining the facet number in theDrosophila mutantBar. Genetics24, 494–508 (1939)
Bownes, M.: Adult deficiencies and duplications of head and thracic structures resulting from microcautery of blastoderm stageDrosophila embryos. J. Embryol. Exp. Morphol.34, 33–54 (1975)
Bryant, P.J.: Regeneration and duplication following operationin situ on the imaginal discs ofDrosophila melanogaster. Dev. Biol.26, 637–651 (1971)
Bryant, P.J.: Pattern formation in the imaginal wing disc ofDrosophila melanogster: fate map, regeneration and duplication. J. Exp. Zool.193, 49–78 (1975)
Bryant, P.J., Adler, P.N., Duranceau, C., Fain, M.J., Glenn, S., Hsei, B., James, A.A., Littlefield, C.L., Reinhardt, C.A., Strub, S., Schneiderman, H.A.: Regulative interactions between cells from different imaginal disks ofDrosophila melanogaster. Science201, 928–930 (1978)
Chan, L.-N., Gehring, W.: Determination of blastoderm cells inDrosophila melanogaster. Proc. Natl. Acad. Sci. USA68, 2217–2221 (1971)
Duranceau, C.: Control of growth and pattern formation in the imaginal wing disc ofDrosophila melanogaster. Ph.D.Thesis, University of California, Irvine: 1977
French, V., Bryant, P.J., Bryant, S.V.: Pattern regulation in epimorphic fields. Science193, 969–981 (1976)
Fullilove, S.L., Jacobson, A.: Embryonic Development — Descriptive. In: The genetics and biology ofDrosophila, 2c (M. Ashburner and T.R.F. Wright, eds.), New York, London: Academic Press 1979, in press
Garcia-Bellido, A., Ripoll, P., Morata, G.: Developmental compartmentalization of the wing disc inDrosophila. Nature New Biol.245, 251–253 (1973)
Garcia-Bellido, A., Ripoll, P., Morata, G.: Developmental compartmentalization of the wing disc inDrosophila. Nature New Biol.245, 251–253 (1973)
Garcia-Bellido, A., Ripoll, P., Morata, G.: Developmental segregations in the dorsal mesothoracic disc ofDrosophila. Dev. Biol.48, 132–149 (1976)
Gateff, E., Schneiderman, H.A.: Developmental capacities of immature eye-antennal imaginal discs ofDrosophila melanogaster. Wilhelm Roux' Archiv176, 171–189 (1975)
Gehring, W.: The stability of the determined state in cultures of imaginal discs inDrosophila. In: Results and problems in cell differentiation (H. Ursprung, R. Nöthiger, eds.), Vol. 5, pp. 35–58. Berlin, Heidelberg, New York: Springer 1972
Gehring, W., Nöthiger, R.: The imaginal discs ofDrosophila. In: Developmental systems: Insects (S.J. Counce and C.H. Waddington, eds.), Vol 2, pp. 211–290. New York, London: Academic Press 1973
Geigy, R.: Erzeugung rein imaginaler Defekte durch ultraviolette Eibestrahlung beiDrosophila melanogaster. Wilhelm Roux' Archiv125, 406–447 (1931)
Hadorn, E., Hurlimann, R., Mindek, G., Schubiger, G., Staub, M.: Entwicklungsleistungen embryonaler Blasteme vonDrosophila nach Kultur im Adultwirt. Rev. Suisse Zool.75, 557–569 (1968)
Haynie, J.L., Bryant, P.J.: Intercalary regeneration in imaginal wing disc ofDrosophila melanogaster. Nature259, 759–762 (1976)
Haynie, J.L., Bryant, P.J.: The effects of X-rays on the proliferation dynamics of cells in the imaginal wing disc ofDrosophila melanogaster. Wilhelm Roux's Archives183, 85–100 (1977)
Illmensee, K., Mahowald, A.: Transplantation of posterior polar plasm inDrosophila. Induction of germ cells at the anterior pole of the egg. Proc. Natl. Acad. Sci. USA71, 1016–1090 (1974)
Illmensee, K., Mintz, B.: Totipotency and normal differentiation of single teratocarcinoma cells cloned by injection into blastocysts. Proc. Natl. Acad. Sci. USA73, 549–553 (1976)
Kauffman, S.: Control circuits for determination and transdetermination. Science181, 310–318 (1973)
Kauffman, S., Shymko, R.M., Trabert, K.: Control of sequential compartment formation inDrosophila. Science199, 259–270 (1978)
Lindsley, D., Grell, E.H.: Genetic variations ofDrosophila melanogaster. Carnegie Inst. Washington Publ. No. 627 (1968)
Madhavan, M.M., Schneiderman, H.A.: Histological analysis of the dynamics of growth of imaginal discs and histoblast nests during the larval development ofDrosophila melanogster. Wilhelm Roux's Archives183, 269–305 (1977)
Mindek, G., Nöthiger, R.: Parameters influencing the acquisition of competence for metamorphosis in imaginal discs ofDrosophila. J. Insect Physiol.19, 1711–1720 (1973)
Mintz, B., Illmensee, K.: Normal genetically mosaic mice produced from malignant teratocarcinoma cells. Proc. Natl. Acad. Sci. USA72, 3585–3589 (1975)
Morata, G. and Garcia-Bellido, A.: Behavior in aggregates of irradiated imaginal disc cells ofDrosophila. Wilhelm Roux' Archiv172, 187–195 (1973)
Nöthiger, R.: The larval development of imaginal discs. In: The biology of imaginal discs (H. Ursprung and R. Nöthiger, eds). Berlin, Heidelberg, New York: Springer 1972
Postlethwait, J.H., Schneiderman, H.A.: Pattern formation in imaginal discs ofDrosophila melanogaster after irradiation of embryos and young larvae. Dev. Biol.32, 345–360 (1973)
Poulson, D.: Histogenesis, organogenesis, and differentiation in the embryo ofDrosophila melanogaster Meigen. In: The biology ofDrosophila (M. Demerec, ed.). New York: Hafner 1950
Reinhardt, C.A., Hodgkin, N.M., Bryant, P.J.: Wound healing in the imaginal discs ofDrosophila. I. Scanning electron microscopy of normal and healing wing discs. Dev. Biol.60, 298–304 (1977)
Schubiger, G.: Acquisition of differentiative competence in the imaginal leg disc ofDrosophila. Wilhelm Roux' Archiv174, 303–311 (1974)
Shearn, A., Rice, T., Garen, A., Gehring, W.: Imaginal disc abnormalities in lethal mutants ofDrosophila. Proc. Natl. Acad. Sci. USA68, 2594–2598 (1971)
Steiner, E.: Establishment of compartments in the developing leg imaginal discs ofDrosophila melanogaster. Wilhelm Roux's Archives180, 9–30 (1976)
Strub, S.: Developmental potentials of the cells of the male foreleg disc ofDrosophila. II. Regulative behavior of dissociated fragments. Wilhelm Roux's Archives182, 75–92 (1977)
Wieschaus, E., Gehring, W.: Clonal analysis of primordial disc cells in the early embryo ofDrosophila melanogaster. Dev. Biol.50, 249–263 (1976)
Wilcox, M., Smith, R.J.: Regenerative interactions betweenDrosophila imaginal discs of different types. Dev. Biol.60, 287–297 (1977)
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Fain, M.J., Schneiderman, H.A. Regulative interaction of mature imaginal disc Fragments with embryonic and immature disc tissues inDrosophila . Wilhelm Roux' Archiv 187, 1–11 (1979). https://doi.org/10.1007/BF00848162
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DOI: https://doi.org/10.1007/BF00848162