Summary
Collagen fibrils with a main period banding of 610 Å and 220 Å in width were observed in the blastocoel of 72-h embryos of the sea urchin,Strongylocentrotus purpuratus. Non-striated fibrils of 50 Å diameter were also observed. The collagen is seen in highest concentration in the vicinity of mesenchyme cells which are richly endowed with endoplasmic reticulum and secretory vesicles. A role for collagen in cell attachment, orientation and spicule formation is discussed.
References
Anderson, H.C., and Griner, S.A.: Cartilage induction in vitro. Dev. Biol.60, 351–358 (1977)
Gibbins, J., Tilney, L., Porter, K.: Microtubules in the formation and development of the primary mesenchyme inArbacia punctulata. I. The distribution of microtubules. J. Cell Biol.41, 201–226 (1969)
Golob, R., Chetsanga, C., Doty, P.: The onset of collagen, synthesis in sea urchin embryos. Biochem, Biophys. Acta349, 135–141 (1974)
Gould, D., Benson, S.C.: Selective inhibition of collagen synthesis in sea urchin embryos by a low concentration of actinomycin D. Exp. Cell Res.112, 73–78 (1978)
Gustafson, T., Wolpert, L.: Cellular movement and contact in sea urchin morphogenesis. Biol. Rev.42, 442–498 (1967)
Hay, E.: Origin and role of collagen in the embryo. Am. Zool.13, 1085–1107 (1973)
Hay, E., Revel, J.: Fine structure of the developing avian cornea. In Monographs in Developmental Biology (A. Wolski and P. Chen, eds.), Vol.1, p. 34, Basel: Karger 1969
Kelafides, N.: Structure and biosynthesis of basement membranes. Int. Rev. Connect. Tissue Res.6, 63–104 (1973)
Lipton, B.: Collagen synthesis by normal and bromodeoxyuridine-modulated cells in myogenic culture. Dev. Biol.61, 153–165 (1977)
Okazaki, K.: Skeleton formation of sea urchin larvae. II. Organic matrix of the spicule. Embryologia5, 283–320 (1960)
Okazaki, K.: Skeleton formation of sea urchin larvae. V. Continuous observation of the process of matrix formation. Exp. Cell Res.40, 585–596 (1965)
Okazaki, K., Inoué, S.: Crystal property of the larval sea urchin spicule. Develop. Growth Differ.18, 413–434 (1976)
Piez, K.: The regulation of collagen fibril formation. In Extracellular Matrix Influences on Gene Expression (H. Slavkin and R. Greulich, eds.), pp. 231–236. New York: Academic Press 1975
Pucci-Minafra, I., Casano, C., LaRosa, C.: Collagen, synthesis and spicule formation in sea urchin embryos. Cell Differ.1, 157–165 (1972)
Pucci-Minafra, I., Minafra, S., Gianguzza, F., Casano, C.: Amino acid composition of collagen extracted from the spicules of sea urchin embryos (Paracentrotus lividus). Bull. Zool.42, 201–204 (1975)
Spurr, A.: A low viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res.26, 31–43 (1969)
Tilney, L., Gibbins, J.: Microtubules in the formation of the primary mesenchyme inArbacia punctulata II. An experimental analysis of their role in development and maintenance of cell shape. J. Cell Biol.41, 227–250 (1969)
Trelstad, R.: Collagen fibrillogenesis in vitro and in vivo: The existence of unique aggregates and the special state of the fibril end. In Extracellular Matrix Influences on Gene Expression (H. Slavkin and R. Greulich, eds.), pp. 331–339. New York: Academic Press 1975
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Crise-Benson, N., Carl Benson, S. Ultrastructure of collagen in sea urchin embryos. Wilhelm Roux' Archiv 186, 65–70 (1979). https://doi.org/10.1007/BF00848108
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DOI: https://doi.org/10.1007/BF00848108