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Kinetics of RNA-synthesis in the 16-cell stage of the sea urchinParacentrotus lividus

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Summary

The concentration of RNA synthesised in the prolonged interphase of micromeres at the 16 cell stage is about 9 times higher per unit volume than in the other blastomeres. Since there are cytoplasmic connections between micromeres and macromeres, a transfer of the micromere RNA by simple diffusion is to be expected.

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References

  • Agrell, I.: A cytoplasmic production of ribonucleic acid during the cell cycle of the micromeres in the sea urchin embryo. Arkiv f. Zool. (2), 435–440 (1958)

  • Bockstahler, L.E.: Incorporation of uridine in cleavage stage eggs of the sea urchinParacentrotus lividus. Z. Naturforsch.26b 816–821 (1971)

    Google Scholar 

  • Cowden, R.R., Lehman, H.E.: A cytochemical study of differentiation in early echinoid development. Growth27, 185–197 (1963)

    PubMed  Google Scholar 

  • Czihak, G.: Entwicklungsphysiologische Untersuchungen an Echiniden. (Ribonucleinsäure-Synthese in den Mikromeren und Entodermdifferenzierung. Ein Beitrag zum Problem der Induktion). Roux' Arch.156, 504–524 (1965)

    Google Scholar 

  • Czihak, G.: Molekularbiologische Aspekte der frühen Embryonalentwicklung von Seeigeln. Publ. Staz. Zool. Napoli,36, 321–345 (1968)

    Google Scholar 

  • Czihak, G., Hörstadius, S.: Transplantation of RNA-labeled micromeres into animal halves of sea urchin embryos. A contribution to the problem of embryonic induction. Develop. Biol.22, 15–30 (1970)

    PubMed  Google Scholar 

  • Czihak, G., Wittmann, H.G., Hindenach, I.: Uridineinbau in die Nucleinsäuren von Furchungsstadien der Eier des SeeigelsParacentrotus lividus. Z. Naturforsch.22b, 1176–1182 (1967)

    Google Scholar 

  • Doermer, P.: In: Micromethods in molecular biology. Chapt. 11: Quantitative autoradiography at the cellular level. (ed. V. Neuhoff), 347–393. Springer Verlag (1973)

  • Hagström, B.E., Lönning, S.: Studies on cleavage and development of isolated sea urchin blastomeres Sarsia18, 1–9 (1965)

    Google Scholar 

  • Hagström, B.E., Lönning, S.: Time-lapse and electron microscopic studies of sea urchin micromeres. Protoplasma68 271–288 (1969)

    PubMed  Google Scholar 

  • Hynes, R.O., Greenhouse, G.A., Minkoff, R., Gross, P.R.: Properties of the three cell types in sixteen-cell sea urchin embryos: RNA synthesis. Develop. Biol.27, 457–478 (1972)

    PubMed  Google Scholar 

  • Hörstadius, S.: Über die Determination im Verlaufe der Eiachse bei Seeigeln. Publ. Staz. Zool. Napoli14, 251–479 (1935)

    Google Scholar 

  • Lönning, S., Hagström, B.E.: Cleavage and differentiation in the sea urchin embryo. Transplantation studies of micromeres. Protoplasma73, 303–322 (1971)

    PubMed  Google Scholar 

  • Mizuno, S., Lee, Y.R., Whiteley, A.H., Whiteley, H.R.: Cellular distribution of RNA populations in 16-cell stage embryos of the sand dollar,Dendraster excentricus. Develop. Biol.37, 18–27 (1974)

    PubMed  Google Scholar 

  • Pucci-Minafra, J., Bosco, M., Giambertone, L.: Preliminary observations on the isolated micromeres from sea urchin embryos. Exptl. Cell Res.53, 177–183 (1968)

    PubMed  Google Scholar 

  • Schmekel, L.: Elektronenmikroskopie oder Makromeren-Mikromerengrenze des Seeigelkeimes. Zool. Anz. Suppl.33, 141–144 (1969)

    Google Scholar 

  • Schmekel, L.: In: The Sea Urchin Embryo (Biochemistry and morphogenesis). Chapt. 9: Egg and embryo ultrastructure. (ed. G. Czihak), 267–307. Springer-Verlag (1975)

  • Spiegel, M., Rubinstein, N.A.: Synthesis of RNA by dissociated cells of the sea urchin embryo. Exptl. Cell Res.70, 423–430 (1972)

    PubMed  Google Scholar 

  • Whiteley, H.R., Mizuno, S., Lee, Y.R., Whiteley, A.H.: Transcripts of reiterated DNA sequences in the determination of blastomeres and early differentiation in echinoid larvae. Americ. Zool.15, 629–648 (1975)

    Google Scholar 

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Czihak, G. Kinetics of RNA-synthesis in the 16-cell stage of the sea urchinParacentrotus lividus . Wilhelm Roux' Archiv 182, 59–68 (1977). https://doi.org/10.1007/BF00848087

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  • DOI: https://doi.org/10.1007/BF00848087

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