Abstract
A method for labeling monoclonal antimyosin antibodies with66Ga is described. The radionuclide is a positron emitter (t1/2: 9.4 h) produced by means of the63Cu (4He, n)66Ga reaction. Purification of the66Ga from the copper target is described in detail. Monoclonal antimyosin antibodies are labeled with66Ga at a high yield (99%) by transcomplexation from an acetate buffer with the diethylenetriamine pentaacetic acid (DTPA)-labeled antibody and preliminary evaluated in two dogs for imaging of myocardial necrosis by positron emission tomography.
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Goethals P, Coene M, Slegers G, Agon P, Deman J, Schelstraete K (1987) Cyclotron production of66Ga to label serum albumin colloids for imaging by PET. Abstract European Nuclear Medicine Congress 1987. Nucl Med 26:171–172
Goethals P, Coene M, Slegers G, Agon P, Deman J, Schelstraete K (1988) Cyclotron production of carrier-free66Ga as a positron emitting label of albumin colloids for clinical use. Eur J Nucl Med 14:152–154
Heyndrickx GR, Amano J, Patrick T, Manders T, Rogers G, Rosendorff C, Vatner SF (1985) Effects of coronary artery reperfusion on regional myocardial blood flow and function in conscious baboons. Circulation 71:1029–1037
Hnatowich DJ (1975) Labeling of tin-soaked albumin microspheres with68Ga. J Nucl Med 17:57–60
Hoberg E, Eisenhut M, Hofmann M, Schuler G, Brachmann J, Aidonidis I, Nachtkamp J, Kübler W, Katus HA (1988) Monoclonal antibodies specific for human cardiac myosin: selection, characterization and experimental myocardial infarct imaging. Eur Heart J 9:328–336
Khaw BA, Fallon JT, Katus H, Elmaleh D, Strauss HW, Locke E, Pohost GM, Haber E (1979a) Positron imaging of experimental myocardial infarction with68Ga-DTPA-anti-myosin antibody. Circulation 135:59–60
Khaw BA, Fallon JT, Beller GA, Haber E (1979b) Specificity of localization of myosin specific antibody fragments in experimental infarction: histologic, histochemical, autoradiographic and scintigraphic studies. Circulation 60:1527–1531
Khaw BA, Fallon JT, Strauss HW, Haber E (1980) Myocardial infarct imaging of antibodies to canine cardiac myosin with Indium-111-diethylenetriamine pentaaectic acid. Science 209:295–297
Phelps ME, Hoffmann EJ, Selin CE, Huang SC, Robinson G, MacDonald N, Schelbert HR, Kuhl DE (1978) Investigation of18F-2-fluoro-2-deoxyglucose for the measure of myocardial glucose metabolism. J Nucl Med 19:1311–1319
Schön HR, Schelbert HR, Robinson GR, Najafi A, Huang SC, Hansen H, Barrio JR, Kuhl DE, Phelps ME (1982) C-11 labeled palmitic acid for the noninvasive evaluation of regional myocardial fatty acid metabolism with positron-computed tomography: I. Kinetics of C-11 palmitic acid in normal myocardium. Am Heart J 103:532–547
Stöcklin G (1980) 3-(11C)-methyl glucose, a promising agent for in vivo assessment of function of myocardial cell membrane. J Nucl Med 21:56–57
Zweit J, Sharma H, Downey S (1987) Production of Gallium-66, a short-lived, positron emitting radionuclide. Int J Appl Radiat Isot 38:499–501
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Goethals, P., Coene, M., Slegers, G. et al. Production of carrier-free66Ga and labeling of antimyosin antibody for positron imaging of acute myocardial infarction. Eur J Nucl Med 16, 237–240 (1990). https://doi.org/10.1007/BF00842774
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DOI: https://doi.org/10.1007/BF00842774