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Affinity chromatography on heparin-sepharose under reducing conditions as a method of selective enrichment with individual isoforms of apolipoprotein E

  • Biophysics and Biochemistry
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Bulletin of Experimental Biology and Medicine Aims and scope

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Literature Cited

  1. A. N. Klimov, K. A. Kozhevnikova, N. N. Klyueva, et al., Mol. Biol.,18, 404 (1984).

    Google Scholar 

  2. L. A. Osterman, Investigation of Biological Macromolecules by Isoelectric Focusing, Immunoelectrophoresis, and Radioisotope Methods [in Russian], Moscow (1983).

  3. Affinty Chromatography. Principles and Methods, Pharmacia Fine Chemicals (1978).

  4. E. N. Avila, G. Holdsworth, N. Sasaki, et al., J. Biol. Chem.,257, 5900 (1982).

    PubMed  Google Scholar 

  5. T. Bitter and H. M. Muir, Analyt. Biochem.,4, 330 (1962).

    PubMed  Google Scholar 

  6. A. Frank, F. A. Shelburne, and S. H. Quarfordt, J. Clin. Invest.,60, 944 (1977).

    PubMed  Google Scholar 

  7. R. E. Greeg, G. Ghiselli, and H. B. Brewer, Jr., Biochim. Biophys. Acta,794, 333 (1984).

    PubMed  Google Scholar 

  8. U. K. Laemmli, Nature,227, 680 (1970).

    PubMed  Google Scholar 

  9. O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, J. Biol. Chem.,193, 265 (1951).

    PubMed  Google Scholar 

  10. P. W. Mahley and T. L. Innerarity, Biochim. Biophys. Acta,737, 197 (1983).

    PubMed  Google Scholar 

  11. R. W. Mahley, T. L. Innerarity, S. C. Rall, Jr., and K. H. Weisgraber, J. Lipid Res.,25, 1277 (1984).

    PubMed  Google Scholar 

  12. F. A. Shelburne and S. H. Quarfordt, J. Biol. Chem.,249, 1428 (1974).

    PubMed  Google Scholar 

  13. G. Utermann, Hoppe-Seyler's Z. Physiol. Chem.,356, 1113 (1975).

    PubMed  Google Scholar 

  14. V. Y. Zannis and J. L. Breslow, Biochemistry,20, 1033 (1981).

    PubMed  Google Scholar 

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Dergunov, A.D., Aniskovich, L.P. & Shuvaev, V.V. Affinity chromatography on heparin-sepharose under reducing conditions as a method of selective enrichment with individual isoforms of apolipoprotein E. Bull Exp Biol Med 110, 894–898 (1990). https://doi.org/10.1007/BF00839921

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  • DOI: https://doi.org/10.1007/BF00839921

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