Theoretical and Applied Genetics

, Volume 86, Issue 4, pp 518–525 | Cite as

Storage-protein variation in wild emmer (Triticum turgidum ssp.dicoccoides) from Jordan and Turkey.

II. Patterns of allele distribution
  • M. Ciaffi
  • D. Lafiandra
  • E. Porceddu
  • S. Benedettelli


Genetic diversity in the seed storage-proteins encoded at theGlu-A1,Glu-B1 andGli-B1/Glu-B3 loci was studied electrophoretically in 315 individuals belonging to nine populations ofT. dicoccoides from Jordan and three from Turkey. The inter- and intra-population distribution of seed storage-protein alleles at the considered loci and its link with geographical factors were investigated. Population differentiation in seed storage-proteins was in some cases very high with very weak correlations with geographic distance. Greater gene differentiation was found within and between populations which were geographically very close in Jordan than between those from Jordan and Turkey. However the distribution of alleles appeared to be non random. Samples collected from populations at locations over 900 m above sea level were less polymorphic than those collected at lower altitudes (500–700 m), whereas the relative genetic differentiation between populations was greater between those collected at higher altitudes. Seed storage-protein differentiation was significantly correlated with the altitude of the collecting sites. Although it is difficult to point out the selective pressure of altitude per se, altitude can reflect an integration of several environmental parameters. The possible adaptive value of seed storage-proteins is discussed.

Key words

Triticum turgidum ssp.dicoccoides Seed storage-protein polymorphism Geographical pattern Adaptive value 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bushuk W, Zillmann RR (1978) Wheat cultivar identification by gliadin electrophoregrams. I. Apparatus, method, and nomenclature. Can J Plant Sci 58:505–515Google Scholar
  2. Cavalli-Sforza LL, Edwards AW (1967) Phylogenetic analyses: models and estimation procedures. Evolution 32:550–570Google Scholar
  3. Ciaffi M, Lafiandra D, Porceddu E, Benedettelli S (1993) Storage protein variation in wild emmer (Triticum turgidum sspdicoccoides) from Jordan and Turkey. I. Electrophoretic characterization of genotypes. Theor Appl GenetGoogle Scholar
  4. Feldman M, Sears ER (1981) The wild gene resources of wheat. Sci Am 244:102–112Google Scholar
  5. Fernandez-Calvin B, Orellana J (1990) High-molecular-weight glutenin subunit variation in theSitopsis section ofAegilops. Implication for the origin of the B genome of wheat. Heredity 65:455–463Google Scholar
  6. Golemberg EM (1986) Multilocus structures in plant populations: population and genetic dynamics ofTriticum dicoccoides. PhD thesis, State University of New York at Stony BrookGoogle Scholar
  7. Jaradat AA, Humeid BO (1990) Morphological variation inTriticum dicoccoides from Jordan. In: Srivastava JP, Damania AB (eds) Wheat genetic resources: meeting diverse needs. Aleppo, Syria, pp 215–222Google Scholar
  8. Kreis M, Shewry PR, Forde BG, Miflin BJ (1985) Structure and evolution of seed storage-proteins and their genes with particular reference to those of wheat, barley and rye. Oxford Surveys Plant Mol Cell Biol 2:253–317Google Scholar
  9. Ladizinsky G (1983) Study of evolutionary problems by means of seed proteins electrophoresis. In: Gottschalk W, Muller HP, Nijhoff M (eds) Seed proteins, biochemistry, genetics, nutritive value. Junk, The Hague Boston London, pp 481–498Google Scholar
  10. Lafiandra D, Benedettelli S, Margiotta B, Spagnoletti-Zeuli PL, Porceddu E (1990) Seed storage-proteins and wheat genetic resources. In: Srivastava JP, Damania AB (eds) Wheat genetic resources: meeting diverse needs. Aleppo, Syria, pp 73–87Google Scholar
  11. Lafiandra D, Masci S, D'Ovidio R, Tanzarella OA, Porceddu E, Margiotta N (1992) Relationship between the D genome of hexaploid wheats (AABBDD) andAe. squarrosa as deduced by seed storage-proteins and molecular-marker analyses. Hereditas 116:233–238Google Scholar
  12. Levy AA, Galili G, Feldman M (1988) Polymorphism and genetic control of high-molecular-weight glutenin subunits in wild tetraploid wheatTriticum turgidum var.dicoccoides. Heredity 61:63–72Google Scholar
  13. Mantel NA (1967) The detection of disease clustering and a generalized regression approach. Cancer Res 27:209–220PubMedGoogle Scholar
  14. Marshall DR, Brown AHD (1975). Optimum sampling strategies in genetic conservatin. In: Frankel OH, Hawkes JG (eds) Crop genetic resources for today and tomorrow. Cambridge University Press, Cambridge, pp 53–70Google Scholar
  15. Nei M (1972) Genetic distances between populations. Am Nat 106:283–292Google Scholar
  16. Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New YorkGoogle Scholar
  17. Nevo E, Payne PI (1987) Wheat storage proteins: diversity of HMW-glutenin subunits in wild emmer from Israel. 1. Geographical patterns and ecological predictability. Theor Appl Genet 74:827–836Google Scholar
  18. Nevo E, Beiles A (1989) Genetic diversity of wild emrner wheat in Israel and Turkey. Structure, evolution, and application in breeding. Theor Appl Genet 77:421–455Google Scholar
  19. Nevo E, Golemberg E, Beiles A, Brown ADH, Zohary D (1982) Genetic diversity and environmental associations of wild wheat,T. dicoccoides in Israel. Theor Appl Genet 62:241–254Google Scholar
  20. Nevo E, Beiles A, Kaplan D (1988) Genetic diversity and environmental associations of wild emmer wheat in Turkey. Heredity 61:31–45Google Scholar
  21. Ohta T, Cockerham CC (1974) Detrimental genes with partial selfing and effects on a neutral locus. Genet Res 23:191–200PubMedGoogle Scholar
  22. Poyarkova H (1988) Morphology, geography and infraspecific taxonomics ofTriticum dicoccoides Korn. A retrospective of 80 years of research. Euphytica 38:11–23CrossRefGoogle Scholar
  23. Rohlf FJ (1987) NTSYS-pc, Numerical Taxonomy and Multivariate Analysis System for the IBM PC microcomputer and compatibles, Exeter Software, Setauket NY, USAGoogle Scholar
  24. Rohlf FJ, Fisher DL (1968) Test for hierarchical structure in random data sets. Systematic Zool 17:407–412Google Scholar
  25. STATGRAPHICS, (STSC) (1987) Statistical Graphics System by Statistical Graphics Corporation, User's Guide, version 3 ed. STSC Inc. NY, USAGoogle Scholar
  26. Thompson G (1977) The effect of a selected locus on linked neutral loci. Genetics 85:753–788PubMedGoogle Scholar
  27. Wright S (1965) The interpretation of population structure by F-statistics with special regard to system of matings. Evolution 19:395–420Google Scholar
  28. Wright S (1978) Evolution and the genetics of populations, vol. 4. Variability within and among natural populations. University of Chicago Press, Chicago, pp 220–246Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • M. Ciaffi
    • 1
  • D. Lafiandra
    • 1
  • E. Porceddu
    • 1
  • S. Benedettelli
    • 2
  1. 1.Department of Agrobiology and AgrochemistryUniversity of TusciaViterboItaly
  2. 2.Agroforestry Institute, C.N.R.Porano (Terni)Italy

Personalised recommendations