The ammonia and glutamine content of brain tissue during anemia resulting from ligation of the carotid arteries
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The author studied the effect of oxygen insufficiency occuring in the brain in “exclusion” of both common carotid arteries on the content of ammonia and glutamine in it. Ammonia was determined by the Balakhovskii-Bruns method modified by us for brain tissue, while amide nitrogen of glutamine was determined by ammonia content after 10-minute hydrolysis with 2 N H2SO4 at 100°C.
The brain tissue of white rats reacted to anemia by widespread stable inhibition. Increase in the quantity of ammonia by 50%, as compared to control, was revealed in the central nervous system. The glutamine content tends to decrease. These changes were more pronounced in the brain tissue of rats in which brain anemia is associated with attacks of general excitation and cramps. The quantity of ammonia in this case increased 3 times, while the amount of glutamine is decreased by 30–35% in comparison with control values.
KeywordsOxygen Nitrogen Ammonia Nervous System Central Nervous System
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