Abstract
A method of titration of choleragen in tadpoles ofRana temporaria is suggested. Unlike in methods known hitherto, the animals are kept (10 at a time) in vessels with water containing serial dilutions of choleragen. The degree of activity of the choleragen is determined from the percentage of dying animals. The dilution of cholergen causing death of 50% of tadpoles after 24 h was taken as the unit of activity. The method is simple, accurate, easily reproducible, and economical in use.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
A. P. Avtsyn, V. A. Shakhlamov, and O. F. Sageeva, Arkh. Pat., No. 3, 41 (1973).
L. F. Zykin, and G. S. Dunaev, Zh. Mikrobiol., No. 3, 52 (1974).
Ya. M. Kabak, A Textbook of Practical Endocrinology [in Russian], Moscow (1945), p. 93.
W. Burrows and G. Musteikis, J. Infect. Dis.,116, 183 (1966).
J. Craig, J. Bact.,92, 793 (1966).
R. Finkelstein, H. Norris, and N. Dutta, J. Infect. Dis.,114, 203 (1964).
A. Lewis and B. Freeman, Science,165, 808 (1969).
R. Finkelstein, cited by L. F. Zykin: “Cellular structures and toxins of the cholera vibrio”, Doctoral Dissertation, Saratov (1971).
V. Watanabe, W. F. Vermey, J. C. Gukian, et al., Texas Rep. Biol. Med.,27, Suppl. 1, 275 (1969).
Author information
Authors and Affiliations
Additional information
Deceased.
Institute of Human Morphology, Academy of Medical Sciences of the USSR. Central Postgraduate Medical Institute, Moscow. Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 80, No. 11, pp. 57–60, November, 1975.
Rights and permissions
About this article
Cite this article
Ermol'eva, Z.V., Avtsyn, A.P., Shakhlamov, V.A. et al. Determination of the activity of choleragen on tadpoles. Bull Exp Biol Med 80, 1329–1331 (1975). https://doi.org/10.1007/BF00835178
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00835178