Immunologic specificity, nature, and properties of suppressor cells induced in mixed lymphocyte cultures
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After incubation of lymphocytes 1–5 days in allogeneic or syngeneic one-way mixed lymphocyte culture (MLC) suppressor cells blocking proliferation of 10 to 100 times as many syngeneic responding lymphocytes in fresh allogeneic MLC are generated. Neither “allogeneic” nor “syngeneic” suppressors have the properties either of B-cells or macrophages, both are resistant to mitomycin C, but their precursors are sensitive to cyclophosphamide. They differ in their properties both from T-killers and from T-suppressors induced in a lymphocyte monoculture. The “allogeneic” suppressors are heterogeneous T-cells as regards radiosensitivity and specificity. The formation of nonspecific T-suppressors is considerably reduced if embryonic calf serum in the medium is replaced by human or bovine serum. “Syngeneic” suppressors also are heterogeneous and differ from “allogeneic” in their greater resistance to anti-T-antibodies and irradiation, whereas their precursors are more resistant to hydrocortisone.
Key Wordssuppressors mixed lymphocyte culture activation of DNA synthesis
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