Abstract
Inhibition of blast-transformation of human lymphocytes, induced by phytohemagglutinin (PHA), with the aid of F(ab′)2-fragment of rabbit IgG was studied. Preincubation of lymphocytes with F(ab′)2-fragments for 24 h at 37 and 4°C followed by change of medium and the addition of PHA did not affect the intensity of blast transformation. However, the F(ab′)2-fragment could depress blast-transformation of lymphocytes if removed from the medium 24 and 48 h after the beginning of stimulation. It can be concluded from these results that the F(ab′)2-fragment interacts only with cells activated by the phytomitogen and exhibits its inhibitory action on cells already bound with PHA.
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Margulis, G.U., Baranova, F.S. & Kul'berg, A.Y. Preliminary activation of lymphocytes with mitogen as the condition for manifestation of ability of Fab-fragments of normal IgG to inhibit lymphocyte transformation. Bull Exp Biol Med 87, 168–170 (1979). https://doi.org/10.1007/BF00833980
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DOI: https://doi.org/10.1007/BF00833980