Effect of audiogenic seizures on activity of H and M forms of lactate dehydrogenase in neurons and neuroglia in various parts of the central nervous system

Abstract

Cytospectrophotometric analysis showed that the ratio between activities of aerobic H forms of lactate dehydrogenase (LD) and the activity of its anaerobic M forms in cerebral cortical neurons and cerebellar Purkinje cells and also in their glial satellite cells of rats of the Krushinskii-Molodkina and Wistar strains is higher than in motoneurons in the anterior horns of the spinal cord and their perineuronal glia. In Krushinskii-Molodkina rats (with genetically determined high sensitivity to audiogenic seizures) epileptiform audiogenic seizures were accompanied by marked activation of both H and M forms of LD in the cortical neurons but by absence of changes in the perineuronal glia. In the spinal motoneurons, on the other hand, the activity of all forms of LD was unchanged, whereas in the neuroglial cells surrounding these neurons activity of the H forms of LD was definitely increased. During the seizures an increase in activity of M forms of LD was found in the cerebellar Purkinje cells but a decrease in their activity in the glial satellite cells.