Bulletin of Experimental Biology and Medicine

, Volume 80, Issue 4, pp 1193–1194 | Cite as

Hydroxylation in isolated liver cells

  • I. P. Kanaeva
  • A. I. Archakov
Biochemistry and Biophysics
  • 13 Downloads

Abstract

Isolated cells were obtained by using EDTA and mechanical action to dissociate rat liver tissue. The presence of oxidative phosphorylation reactions in the hepatocytes was taken as an indication of their integrity. The hepatocytes were able to hydroxylate dimethylaniline, ethylmorphine, and aminopyrine. The maximal velocity of hydroxylation was higher in the cells than in microsomes, when calculated per nmole cytochrome P-450. The NAD·H2 formed by oxidation of glutamate and malate in isolated cells can be used for the hydroxylation reactions.

Key Words

isolated cells hydroxylation oxidative phosphorylation oxidation 

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Copyright information

© Plenum Publishing Corporation 1976

Authors and Affiliations

  • I. P. Kanaeva
  • A. I. Archakov

There are no affiliations available

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