Abstract
Rat adrenal glands were immersed in Ringer-Locke solution (control) and, in parallel tests, in human blood plasma. After incubation for 2 h the corticosterone concentration in the extracts was determined. Activity of the pituitary adrenocorticotropic function of the patients tested was judged from the difference between the corticosterone values in the experimental and control extracts. To determine the sensitivity of the method ACTH was added to blood plasma in doses of 1, 5, and 10 μg. A significant and regular increase in the corticosterone concentration was found. The modification of Pierre's method is perfectly suitable for the estimation of ACTH in man.
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Literature Cited
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Glebov, B.F., Lazarev, N.I. Grounds for using Pierre's method of ACTH estimation in human blood plasma. Bull Exp Biol Med 79, 475–476 (1975). https://doi.org/10.1007/BF00832733
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DOI: https://doi.org/10.1007/BF00832733