Abstract
As a result of crossing between Hfr H, KL-96, and KL-99 donor cells with AB 2463 rec A as the recipient, merodiploids carrying factors of different structure (different length) and different activity were isolated: 1) typical F' factors with incorporated proximal chromosomal markers; 2) “long” F' factors of different structure, defective for genes controlling sensitivity to phage f2; 3) “long” F' factors of different structure defective for genes controlling transfer. Chromosomal markers can be incorporated into the factor regardless of their position relative to sex factor in the original Hfr cells. Defects of the sex factor proper are accompanied by loss of some of its incorporated chromosomal genes, whereas the typical F' factors preserve their structure completely.
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Pavlovich, A.I. Properties of the F' factors formed in crosses between escherichia coli Hfr donor cells and recombination defective recipients. Bull Exp Biol Med 79, 456–458 (1975). https://doi.org/10.1007/BF00832726
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DOI: https://doi.org/10.1007/BF00832726