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Plant Cell Reports

, Volume 8, Issue 9, pp 517–520 | Cite as

Interspecific somatic hybrids ofRudbeckia hirta andR. Laciniata (Compositae)

  • J. S. Al-Atabee
  • B. J. Mulligan
  • J. B. Power
Article

Abstract

Interspecific somatic hybrid plants betweenRudbeckia hirta cv. Marmalade andR.laciniata cv. Irish Eyes were regenerated following the electro-fusion of mesophyll protoplasts ofR.hirta with callus protoplasts ofR.laciniata. A hybrid selection scheme was based on the fact that plant regeneration, from parental protoplasts ofR.hirta, was via shoot regeneration of callus, and only via rhizogenesis forR.laciniata. The other half of the selection strategy was based on the presence of anthocyanin-pigmented roots; a characteristic of theR.hirta parent only. Somatic hybrids were regenerated, via rhizogenesis, alongside normalR.laciniata but were distinguished by the presence of pigmented roots (a feature ofR.hirta). Hybrid plants had a floral morphology that was intermediate as compared to that of the two parents, with an expected somatic chromosome number of 2n=(2x+4x)=74. Pollen viability though was low. Esterase and peroxidase isozyme profiles confirmed the hybrid nature of the regenerated plants with pigmented roots, whilst chloroplast DNA restriction analysis showed that these hybrids had aR.laciniata chloroplast DNA. This demonstration of somatic hybridisation not only opens up the possibility of incorporating novel traits between such ornamentalCompositae species, but provides a selection strategy based on rhizogenesis as the route to plant regeneration coupled with heritable pigmentation production of roots as a confirmatory hybrid marker.

Keywords

Plant Regeneration Somatic Hybrid Hybrid Plant Pollen Viability Somatic Chromosome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

ABBREVIATIONS

BSA

bovine serum albumin

EDTA

ethylene diamine tetra acetic acid

FDA

fluorescein diacetate

f.wt.

fresh weight

IAA

indole 3-acetic acid

MS

Murashige and Skoog (1962) medium

TEMED

N,N,N′,N′-Tetra methyl ethylene diamine

TES

(N-tris (hydroxymethyl) methyl-2-aminoethanesulfonic acid)

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • J. S. Al-Atabee
    • 1
  • B. J. Mulligan
    • 1
  • J. B. Power
    • 1
  1. 1.Plant Genetic Manipulation Group, Department of BotanyUniversity of NottinghamNottinghamUK

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