On the mechanism of citrate inhibition of ceruloplasmin ferroxidase activity

Abstract

Ceruloplasmin is a plasma protein, which oxidizes ferrous ions in a catalytic manner. It is considered to function as a ferroxidasein vivo. Citrate was found to inhibit the reaction. The ceruloplasmin catalyzed oxidation ofp-phenylenediamines, however, was not affected by citrate. The inhibitory effect is proposed to be due to formation of Fe2+-citrate, which does not react with ceruloplasmin. The stability constant for the Fe2+-citrate complex estimated from the present inhibition study is in good agreement with previously published data.

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Correspondence to Rolf Arthur Løvstad.

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Løvstad, R.A. On the mechanism of citrate inhibition of ceruloplasmin ferroxidase activity. Biometals 9, 273–275 (1996). https://doi.org/10.1007/BF00817927

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Keywords

  • ceruloplasmin
  • citrate
  • ferroxidase
  • iron ions