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Kinetic method for the study of xylan hydrolysis by xylan hydrolases

Eine kinetische Methode zur Untersuchung der Hydrolyse von Xylan durch Xylan-Hydrolase

  • Organische Chemie Und Biochemie
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Summary

The Somogyi-Nelson colorimetric method was used in a new manner more suitable for evaluating the kinetics of the enzyme hydrolysis of xylan catalyzed by xylan hydrolases. The values of the Michaelis parameters (Km=5.56 g l−1 andV=2.94 · 10−5 M s−1) were determined.

Zusammenfassung

Die kolorimetrische Methode nach Somogyi-Nelson wurde nach einem neuen Verfahren zur Verfolgung der Kinetik der hydrolytischen Spaltung von Xylan, katalysiert durch Xylan-Hydrolasen vonAspergillus oryzae, angewandt. Es wurden die Michaelis-Parameter (Km=5.56 g l−1 undV=2.94 · 10−5 M s−1) bestimmt.

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Authors and Affiliations

  1. Faculty of Biology, University of Sofia, 1421, Sofia, Bulgaria

    Nadka G. Bakalova, Methodi L. Chetkarov, Todor K. Nikolov & Dimiter N. Kolev

Authors
  1. Nadka G. Bakalova
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  2. Methodi L. Chetkarov
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  3. Todor K. Nikolov
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  4. Dimiter N. Kolev
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Bakalova, N.G., Chetkarov, M.L., Nikolov, T.K. et al. Kinetic method for the study of xylan hydrolysis by xylan hydrolases. Monatsh Chem 120, 343–348 (1989). https://doi.org/10.1007/BF00811747

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  • DOI: https://doi.org/10.1007/BF00811747

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