Abstract
Under the influence of 1 mM cyclic-adenosine-3′,5′-monophosphate (cyclic-AMP) the degree of survival and rate of reproduction of Chinese hamster cells in culture were reduced to 27 and 42% of the control level, respectively. Addition of 0.02 mM thymidine along with the cyclic-AMP almost completely abolished the cytostatic effect of the latter. Thymidine also prevented the cytostatic effect of noncyclic 5′-AMP, but did not affect death of the cells due to the action of dibutyryl cyclic-AMP and theophylline. Thymidine did not prevent the inhibitory action of cyclic-AMP on a mutant line of mouse cells deficient in thymidine kinase. It is concluded that, in the concentrations used, the cytostatic action of exogenous cyclic-AMP on mammalian cells is the result of its splitting to 5′-AMP in the culture medium, and that it acts by blocking one of the stages of TMP biosynthesis.
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Polunovskii, V.A. Protective effect of thymidine against cytostatic action of cyclic-AMP in mammalian cell cultures. Bull Exp Biol Med 80, 815–817 (1975). https://doi.org/10.1007/BF00809901
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DOI: https://doi.org/10.1007/BF00809901