Abstract
The effectiveness of trypan blue, acridine orange, tetracycline, and oxytetracycline for the detection of tumor cells injected into the bloodstream was investigated in experiments on rats. Cells were detected in the microvessels of the mesentery by intravital microscopy. Luminescence of the fluorochromed cells was observed in blue-violet (λMax 400 nm) and ultraviolet (λMax 365 nm) rays of a mercury vapor lamp and in laser radiation (λ 337 nm). The intensity of luminescence of cells stained with acridine orange was higher, and their structure could be distinguished better, than in cells containing tetracyclines. Identification of the cells by trypan blue was difficult. The luminescence method is sufficiently simple, and it can be used to discover neoplastic cells actually in the bloodstream.
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Laboratory of General Pathology and Experimental Therapy, Institute of General Pathology and Pathological Physiology, Academy of Medical Sciences of the USSR, Moscow. Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 80, No. 7, pp. 79–82, July, 1975.
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Shinkarenko, V.S., Aleksandrov, P.N. & Chernukh, A.A.M. Detection of tumor cells in the blood stream. Bull Exp Biol Med 80, 802–804 (1975). https://doi.org/10.1007/BF00809896
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DOI: https://doi.org/10.1007/BF00809896