Acid phosphatase and succinate and dehydroorotate dehydrogenase activity during interaction between allogeneic lymphocytes and target cells in tissue culture

Abstract

Investigation of acid phosphatase activity during interaction between lymphocytes and target cells showed that its greatest increase is observed in lymphocytes in 1-h and sometimes 3-h cultures, whereas in the cells it was observed after 3–6 h. Initial acid phosphatase activity was higher in immune lymphocytes, but later, on their addition to a culture of L-cells, no significant difference was found between the change in enzyme activity in immune and normal lymphocytes. Acid phosphatase activity in L-cells was lower on the addition of normal than of immune lymphocytes. Activity of the dehydrogenases in the lymphocytes increased until 3 h of incubation and the increase was greater in immune than in normal lymphocytes. Activity of succinate and dehydroorotate dehydrogenases in the L-cells changed at virtually the same times as in the lymphocytes. Increased activity of oxidoreductases and acid phosphatase in the first few hours of contact is evidence of the rapid activation of effector lymphocytes.