Activity of isozymes of NAD- and NADP-dependent malate dehydrogenases in the myocardium of rabbits with alloxan diabetes

  • L. N. Dagaeva
Biochemistry and Biophysics


Isozymes of NAD- and NADP-dependent malate dehydrogenases (NAD- and NADP-MD) isozymes in rabbit heart muscles were detected by disk electrophoresis in polyacrylamide gel. In alloxan diabetes a significant decrease in mitochondrial NADP-MD activity (in the malate decarboxylation reaction) was observed, but its activity in the cytoplasm was increased. NAD-MD activity (in the oxaloacetate reduction reaction) also was lowered in the myocardium in diabetes, especially in the mitochondria. Insulin restored the ratio between the activities of the NAD- and NADP-MD isozymes, disturbed in diabetes, in the subcellular structures of the myocardium.

Key Words

malate dehydrogenase heart-muscle isozymes in diabetes 

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Copyright information

© Plenum Publishing Corporation 1975

Authors and Affiliations

  • L. N. Dagaeva

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