Journal of Bioenergetics and Biomembranes

, Volume 22, Issue 5, pp 679–689 | Cite as

Dicyclohexylcarbodiimide as inducer of mitochondrial Ca2+ release

  • Edmundo Chávez
  • Cecilia Zazueta
  • Enrique Díaz
Research Articles


The effect of the alkylating reagent dicyclohexylcarbodiimide (DCCD) on mitochondrial Ca2+ content was studied. The results obtained indicate that DCCD at a concentration of 100 µM induces mitochondrial Ca2+ efflux. This reaction is accompanied by an increasing energy drain on the system, stimulation of oxygen consumption, and mitochondrial swelling. These DCCD effects can be partially suppressed by supplementing the incubation medium with 1 mM phosphate. By electrophoretic analysis on polyacrylamide-sodium dodecyl sulfate, it was found that DCCD binds to a membrane component with anMr of 20 to 29 kDa.

Key Words

Dicylcohexylcarbodiimide: DCCD: Ca2+ release Kidney mitochondria 


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Copyright information

© Plenum Publishing Corporation 1990

Authors and Affiliations

  • Edmundo Chávez
    • 1
  • Cecilia Zazueta
    • 1
  • Enrique Díaz
    • 1
  1. 1.Departamento de Bioquímica, Instituto Nacional de CardiologíaIgnacio ChávezMexico, D. F.Mexico

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