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Electron-histochemical and biochemical investigation of the glucose-6-phosphatase andβ-glycerophosphatase activities in nuclei of the normal liver and hepatoma-27 of rats

  • Morphology and Pathomorphology
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Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

The presence of glucose-6-phosphatase activity was demonstrated electron-histochemically and biochemically in rat liver cell nuclei, and the histochemical reaction product was localized mainly in the perinuclear space. The activity of this enzyme in hepatoma-27 nuclei was much lower than in nuclei isolated from normal liver. A low level ofβ-glycerophosphatase activity was detected histochemically in liver nuclei incubated at pH 6.4. No activity of this enzyme was found in hepatoma-27 nuclei. Biochemical tests revealed traces of activity in both liver and hepatoma-27 nuclei.

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Bukhvalov, I.B., Koen, Y.M., Perevoshchikov, A.G. et al. Electron-histochemical and biochemical investigation of the glucose-6-phosphatase andβ-glycerophosphatase activities in nuclei of the normal liver and hepatoma-27 of rats. Bull Exp Biol Med 78, 1438–1440 (1974). https://doi.org/10.1007/BF00808728

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  • DOI: https://doi.org/10.1007/BF00808728

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