Summary
The method of two-stage half-specific amplification was described and successfully used in the isolation of the protein-coding part of the thioredoxin gene from Streptomyces aureofaciens BMK. The efficiency of a new PCR modification for the specific amplification of the target DNA fragments (genes) with unknown sequences is compared with the used half-specific PCR. The determined target sequence demonstrates the highest homology with the thioredoxin genes from Corynebacterium nephridii C-1 and Anabaena 7119.
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References
Labudová O, Kollárová M (1991) Thioredoxin: structure, properties, fuctions. Biokhimiya 56: 977–989
Lim C-J, Gleason FK, Futchs JA (1986) Cloning, expression and characterization of theAnabaena thioredoxin gene inE. coli. J Bacteriol 168: 1258–1264
Meng M, Hogenkamp HPC (1981) Purification, characterization and amino acid sequence of thioredoxin fromCorynebacterium nephridii. J Biol Chem 256: 9174–9182
Müller EGD, Buchanan BB (1989) Thioredoxin is essential for photosynthetic growth. J Biol Chem 164: 4008–4014
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Labudová, O., Némethová, M., Kollárová, M. et al. PCR cloning of a protein-coding part of the thioredoxin gene fromStreptomyces aureofaciens . Amino Acids 6, 107–110 (1994). https://doi.org/10.1007/BF00808126
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DOI: https://doi.org/10.1007/BF00808126