Amino Acids

, Volume 6, Issue 1, pp 37–45 | Cite as

The effect of ovariectomy of serum amino acids and cholesterol in the rat

  • G. Frauscher
  • G. Lubec


As steroid hormones are known to influence amino acid metabolism we tested the hypothesis that ovariectomy should lead to significant changes in this system.

We found that after ovariectomy serum alanine was significantly decreased (p = 0.0006) in contrast to serum glycine and branched chain amino acids (BCAA). The ratio of glycine/BCAA, a parameter for anabolism or catabolism was not changed after ovariectomy. If, however, the amino acid alanine as the link to carbohydrate and lipid metabolism was introduced the alanine/BCAA ratio was significantly altered (p = 0.01).

Although serum cholesterol was altered as well (increased,p = 0.03), no significant correlation with alanine was found. We can therefore assume that there are two independent mechanisms for lipid and amino acid changes after ovariectomy.

The most prominent finding was that estradiol replacement after ovariectomy restored increased cholesterol levels but did not restore alanine levels. Other ovarial hormones must be incriminated for the regulation of alanine metabolism. The anabolic effects of estradiol as decreasing glycine and BCAA were noticed which rules out insufficient estradiol replacement.


Amino acids Ovariectomy Estradiol Glycine Alanine BCAA 


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  1. Arroyave G (1970) Comparative sensitivity of specific amino acid ratios vs essential to nonessential' amino acid ratio. Am J Clin Nutr 23: 703–706Google Scholar
  2. Brodin SV, Yanovich VG, Vovk SV. The lipogenic role of leucine alanine and lysine in the rat tissues in vivo. Amino Acids (accepted for publication.)Google Scholar
  3. Felig P, Owen OE, Wahren J, Cahill GF Jr. (1969) Amino acid metabolism during prolonged starvation. J Clin Invest 48: 584–585Google Scholar
  4. Granfone A, Campos H, McNamaa JR, Schaefer MM (1992) Effects oestrogen replacement on plasmalipoproteins and alipoproteins in postmenopausal, dyslipidic women. Metabolism 41: 1193–1198Google Scholar
  5. Holt LE Jr, Snyderman SE, Norton PM, Roitman E (1963) The plasma aminogram in kwashiorkor. Lancet ii: 1343–1348 (Metabolism 41: 1193–1198)Google Scholar
  6. Kameneva I, Shavva AG, Lozovskii VG, Ryzhenkov VE (1992) Changes in blood serum lipids and lipoproteins of rats in ovariectomy and to the administration of the methyl ester of 6-hydroxy-D-homo-8-isostrone. Biull Eksp Biol-AG 114: 148–150Google Scholar
  7. Lindblad BS, Rahimtoola RJ, Rehman HU (1978) Plasma free amino acid levels during the initial rehabilitation of proteine energy malnutrition with protracted diarrhoea using a free amino acid-glucose diet. Acta Paed Scand 67: 335–343Google Scholar
  8. Nozaki M, Hashimoto K, Sumii Y (1993) Changes in bone and lipid metabolisms following oophorectomy and effects of estrogen replacement therapy. Nippon Sanka Fujinka Gakkai Zasshi 45: 38–44Google Scholar
  9. Rosenthal J, Angel A, Farcas J (1974) Metabolic fate of leucine: a significant sterol precursor in adipose tissue and muscle. Am J Physiol 226: 411–418Google Scholar
  10. SAS User's Guide (1985) Statistics. Version 5 Edition: SAS Institute Inc. Cary, NC, 1985Google Scholar
  11. Scholz RW, Evans M (1977) Pulmonary fatty acid synthesis II. Amino acids as fatty acid precursors in rat lung. Am J Physiol 232: 364–369Google Scholar
  12. Seishima M, Bisgaier CL, Davies SL, Glickman RM (1991) Regulation of hepatic apolipoprotein synthesis in the 17 ethinyl estradiol treated rat. J Lipid Res 32: 941–951Google Scholar
  13. Srivastava RA, Tang J, Baumann D, Schonfeld G (1992) Hormonal and nutritional stimuli modulate apolipoprotein B mRNA editing in mouse liver. Biochem Biophys Res Comm 188: 135–141Google Scholar
  14. van-der-Mooren MJ, Demacker PN, Thomas CM, Rolland R (1992) Beneficial effects on serum lipoproteins by 17 beta-estradiol-dydrogesterone therapy in postmenopausal women; a prospective study. Eur J Obstet Gynecol Reprod Biol 47: 153–160Google Scholar
  15. Vernon RG, Finley E, Taylor E (1985) Fatty acid sythesis from amino acids in sheep adipose tissue. Comp Biochem Physiol 82B: 133–136Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • G. Frauscher
    • 1
  • G. Lubec
    • 1
  1. 1.Department of PediatricsUniversity of ViennaViennaAustria

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