Abstract
The mechanism of the technique of bisulfite treatment followed by staining with toluidine and methylene blue, after hydrolysis by Feulgen's method was studied and its suitability for histochemistry assessed. Bisulfite treatment was found to increase the intensity of the reaction by 1.5 times, and binding of the dye takes place stoichiometrically. Toluidine blue gives metachromatic and anisotropic staining of the cell nuclei. The method is recommended as a sensitive test for DNA in cytochemical investigations and for studying dichronism of DNA-containing structures.
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Érenpreisa, E.A. Modification of the method of staining for DNA with basic dyes. Bull Exp Biol Med 83, 596–598 (1977). https://doi.org/10.1007/BF00807519
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DOI: https://doi.org/10.1007/BF00807519