Bulletin of Experimental Biology and Medicine

, Volume 83, Issue 4, pp 591–595 | Cite as

Some structural features of vital dyes

  • M. G. Iogannsen
Morphology and Pathomorphology


Lymphocytes and macrophages of healthy rats, cells of lymphosarcoma and ovarian tumors (strain OYa) of rats, and cells of sarcoma 37 and lymphosarcoma Nk/Ly of mice were stained intravitally with 1·10−5–3·10−2 M solutions of 86 dyes. The presence of alkylating amino groups in the molecule of the dye substantially increases its ability to penetrate to living cells and to be deposited in their cytoplasm. Acid radicals considerably reduce the ability of the dye to stain cells intravitally. This has been shown for thiazine, oxazine, triphenylmethane, acridine, diazine, and xanthene compounds. The degree of basicity of the dye molecule and also of its amino group does not play a decisive role in the process of vital staining.

Key Words

vital dyes intravital microscopy cell membranes permeability 


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Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • M. G. Iogannsen

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