Advertisement

Bulletin of Experimental Biology and Medicine

, Volume 83, Issue 4, pp 555–557 | Cite as

Effect of cultivation temperature on blast transformation of lymphocytes from people of different ages

  • Yu. V. Pakin
Experimental Biology

Abstract

The effect of the cultivation temperature on blast transformation of lymphocytes from persons aged 20–35 and 90–102 years induced by phytohemagglutinin was studied. Cultivation at 39°C was found to increase, but at 41°C to reduce sharply the index of blast transformation compared with the control (37°C). The effect of cultivation temperature on the blast-transformation process has certain features which depend on the donors' age.

Key Words

blast transformation lymphocytes age temperature 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    L. G. Barbaruk, Tsitol. Genet., No. 1, 28 (1974).Google Scholar
  2. 2.
    P. A. Borodkin, Byull. Éksp. Biol. Med., No. 12, 87 (1972).Google Scholar
  3. 3.
    P. A. Borodkin, in: Biological Research in the North-East of the European Part of the USSR [in Russian], Syktyvkar (1974), p. 161.Google Scholar
  4. 4.
    V. P. Voitenko, L. G. Barbaruk, and Yu. V. Pakin, Ter. Arkh., No. 12, 34 (1974).Google Scholar
  5. 5.
    K. M. Geine, R. Klatt, G. German, et al., Probl. Gematol., No. 8, 29 (1970).Google Scholar
  6. 6.
    N. R. Ling, Stimulation of Lymphocytes [in Russian], Moscow (1971), p. 31.Google Scholar
  7. 7.
    P. G. Nazarov, Lab. Delo, No. 2, 74 (1975).Google Scholar
  8. 8.
    Yu. V. Pakin and L. G. Barbaruk, Tsitol. Genet., No. 3, 195 (1976).Google Scholar
  9. 9.
    V. G. Pisarenko, Yu. V. Pakin, and V. N. Starkov, Dokl. Akad. Nauk Ukrainsk. SSR, Ser. B, No. 6, 552 (1976).Google Scholar
  10. 10.
    N. L. Samoilina, Lab. Delo, No. 8, 455 (1970).Google Scholar
  11. 11.
    A. Ya. Fridenshtein and I. L. Chertkov, The Cellular Bases of Immunity [in Russian], Moscow (1969), p. 92.Google Scholar
  12. 12.
    A. S. Coulson and D. G. Chalmers, Nature,209, 378 (1966).Google Scholar
  13. 13.
    H. M. Hallgren, C. E. Buckley, and E. J. Yunis, Gerontologist,13, 46 (1973).Google Scholar
  14. 14.
    O. R. McIntyre and A. F. Cole, Int. Arch. Allergy,35, 105 (1969).Google Scholar
  15. 15.
    M. E. Weksler and T. H. Hutteroth, J. Clin. Invest.,53, 99 (1974).Google Scholar

Copyright information

© Plenum Publishing Corporation 1977

Authors and Affiliations

  • Yu. V. Pakin

There are no affiliations available

Personalised recommendations