Abstract
Biosynthesis of RNA catalyzed by DNA-dependent RNA polymerase was demonstrated in a reconstructed system containing isolated lymphocyte nuclei, Mg2+ or Mn2+ salts, and ammonium sulfate, in the presence of four nucleoside triphosphates. Both Mg2+- and Mn2+-dependent forms of this enzyme were found in the nuclei of normal lymphocytes and of lymphocytes from patients with melanoma, lung carcinoma, and sarcoma. The activity of both forms of RNA polymerase in the nuclei of the patients' lymphocytes was higher than in normal analogs. The sensitivity of DNA-dependent RNA polymerase to dexamethasone and phytohemagglutinin was less marked in the nuclei from patients with lung carcinoma, melanoma, and sarcoma than in normal lymphocytes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
M. N. Blinov, A. D. Vladimirova, and I. S. Luganova, Tsitologiya,16, 1388 (1974).
S. V. Kozhanova, A. K. Kosaev, and Yu. Sh. Kaliev, in: The Immunology of Tumors [in Russian], Kiev (1975), p. 130.
I. S. Luganova and I. F. Seits, Lab. Delo, Nw. 9, 541 (1967).
R. V. Petrov et al., Usp. Sovrem. Biol.,77, No. 1, 90 (1974).
N. N. Trapeznikov, V. I. Kupin, R. M. Radzikhovskaya, et al., Dokl. Akad. Nauk SSSR,222, 468 (1975).
N. M. Turkevich and E. M. Samundzhan, Hormones and Carcinogenesis [in Russian], Kiev (1975), p. 34.
K. Burton, Biochem. J.,62, 315 (1956).
W. Catalana, W. Sample, and P. Chretien, Cancer (Philadelphia),31, 65 (1973).
A. Cooke and J. Kay, Exp. Cell Res.,79, 179 (1973).
S. Handmaker and J. Graef, Biochim. Biophys. Acta,199, 95 (1970).
T. Kanaya, Sapporo Med. J.,43, 48 (1974).
A. Nagel, W. L. Piessens, and T. Stilmant, Eur. J. Cancer,1, 41 (1971).
B. Pogo, J. Cell Biol.,53, 635 (1972).
C. Plotz, A. Knowlton, and C. Ragan, Am. J. Med.,8, 597 (1952).
W. Sealy, Surgery,68, 906 (1970).
H. Spengeler, K. Brunner, and A. Weck, Schweiz, Med. Wochenschr.,102, 1167 (1972).
Author information
Authors and Affiliations
Additional information
Laboratory of Biochemistry of Tumors, All-Union Oncologic Center of the USSR, Moscow. Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 83, No. 4, pp. 449–452, April, 1977.
Rights and permissions
About this article
Cite this article
Ioannesyants, I.A., Adler, V.V., Él'kina, Z.I. et al. Sensitivity of RNA-synthesizing system of the lymphocytes of patients with malignant neoplasms to phytohemagglutinin and dexamethasone. Bull Exp Biol Med 83, 529–532 (1977). https://doi.org/10.1007/BF00807497
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00807497