Amino Acids

, Volume 10, Issue 3, pp 263–271 | Cite as

Cell growth in arginine- and ornithine-deprived medium and conversion of glutamate to ornithine and arginine in rat hepatoma cells

  • K. Yamamoto
  • A. Niwa


A subline growing in medium without arginine and ornithine was established from a rat Reuber hepatoma cell line (R-Y121B·cho). The subline designated R-Y117B·cho was able to grow in glutamine, arginine and ornithine-free, glutamate-supplemented medium. Arginine synthesis from glutamate requires four urea cycle enzymes and another two enzymes, glutamate semialdehyde dehydrogenase and ornithine aminotransferase. Since R-Y121B·cho cells have all the urea cycle enzymes, two other enzyme activities were determined. The activities of ornithine aminotransferase and glutamate semialdehyde dehydrogenase were similar in R-Y117B·cho and its parental R-Y121B·cho cells, but R-Y117B·cho cells had higher conversion of glutamate to arginine than parental cells.

Key words

Amino acids Arginine Cultured cell Glutamate semialdehyde dehydrogenase Ornithine Ornithine aminotransferase 


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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • K. Yamamoto
    • 1
  • A. Niwa
    • 1
  1. 1.Department of MicrobiologyDokkyo University School of MedicineMibu, TochigiJapan

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