Amino Acids

, Volume 1, Issue 2, pp 239–246 | Cite as

d-Aminoaciduria in mutant mice lackingd-amino-acid oxidase activity

  • R. Konno
  • M. Ikeda
  • A. Niwa
  • Y. Yasumura
Article

Summary

Urine of mutant ddY/DAO mice lackingd-amino-acid oxidase activity contained more serine and proline than that of normal ddY/DAO+ mice.d-Amino-acid oxidase treatment of urinary amino acids decreased the serine and proline, suggesting that they containedd-isomers. An HPLC analysis confirmed the presence ofd-serine. Urinary serine and proline contents were not decreased when the ddY/DAO mice were fed a diet which did not contain supplementaryd-methionine or when they were given water containing antibiotics. These results suggest that thed-serine andd-proline do not derive from thed-methionine supplemented in the diet or from intestinal bacteria. In urine of the ddY/DAO mice, a substance which seemed to bed-methionine sulfoxide and/ord-methionine sulfone was present. It is probably a metabolite of thed-methionine supplemented in the diet. Thed-aminoaciduria in the mutant mice lackingd-amino-acid oxidase activity indicates that this enzyme is involved in the metabolism of thed-amino acids in normal mice.

Keywords

Amino acids d-Amino-acid oxidase d-Aminoaciduria Physiological role Mutant mice d-Amino acids Urine 

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • R. Konno
    • 1
  • M. Ikeda
    • 2
  • A. Niwa
    • 1
  • Y. Yasumura
    • 1
  1. 1.Department of MicrobiologyDokkyo University School of MedicineMibu, TochigiJapan
  2. 2.Department of Laboratory of Medical SciencesDokkyo University School of MedicineMibu, TochigiJapan

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