Method of obtaining micropreparations of intestinal villi

Abstract

To obtain reliable microscopic pictures, a method of processing and embedding in paraffin wax, followed by cutting complete series of sections in assigned planes through individual intestinal villi, isolated under the control of a binocular loupe from biopsy specimens of the mucous membrane of the small intestine, fixed with 12% neutral formalin and not attached to any surface, was used. The method ensures adequate evaluation of villi of different shapes both as a whole and in micropreparations.