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Quantitative determination of lysozyme in blood serum

  • Microbiology and Immunology
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

A method of quantitative determination of lysozyme in human and rat blood serum, based on the ability of lysozyme to undergo selective adsorption on chitin with the formation of a reversible enzyme—substrate complex, is suggested. The method is simple in its use and does not require expensive equipment, so that it can be used in clinical practice. Lysozyme was determined by this method quantitatively in human and male rat blood serum, and the results were several times greater than the values of its lytic activity. This shows that the suggested method can be used to obtain additional characteristics of lysozyme besides its lytic activity.

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Babayan, S.S., Zhuravleva, T.P. & Lebedeva, L.L. Quantitative determination of lysozyme in blood serum. Bull Exp Biol Med 87, 446–447 (1979). https://doi.org/10.1007/BF00806678

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  • DOI: https://doi.org/10.1007/BF00806678

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