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Amino Acids

, Volume 10, Issue 2, pp 133–143 | Cite as

Moused-amino-acid oxidase gene: Restriction fragment length polymorphism among mouse strains

  • R. Konno
  • M. Sasaki
  • M. Shinohara
  • J. Enami
  • A. Niwa
Article

Summary

Genomic DNA was extracted from mice of 15 strains (A/J, AKR, BALB/c, C3H/He, C57BL/6, CBA/J, CD-1, CF#1, DBA/2, ddY/DAO+, ddY/DAO, ICR, NC, NZB and NZW) for the examination of the difference in the structure of thed-amino-acid oxidase gene among the mouse strains. The DNAs were digested with restriction endonucleases and analyzed by Southern hybridization usingd-amino-acid oxidase cDNA as a probe. The 15 strains showed the same hybridization patterns in theEcoRV,BamHI orBglII digestion. In theEcoRI digestion, the DBA/2 strain showed a different hybridization pattern from the other 14 strains. In thePvuII andXbaI digestion, C3H/He, CBA/J, ddY/DAO+ and NC strains were different from the other 11 strains. In thePstI andHindIII digestion, restriction fragment length polymorphisms were observed, and the 15 strains were classified into four groups according to their hybridization patterns. These results indicate that the 15 strains of mice carry a structurally similard-amino-acid oxidase gene, but there is a variation in its inside sequence among the groups of the strains.

Keywords

d-Amino-acid oxidase Southern hybridization Mouse strains RFLP Gene structure 

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • R. Konno
    • 1
  • M. Sasaki
    • 2
  • M. Shinohara
    • 3
  • J. Enami
    • 2
  • A. Niwa
    • 1
  1. 1.Department of MicrobiologyDokkyo University School of MedicineMibu, TochigiJapan
  2. 2.Research LaboratoryZenyaku Kogyo Co., Ltd.Oizumi, Nerima, TokyoJapan
  3. 3.Department of Oral SurgeryDokkyo University School of MedicineMibu, TochigiJapan

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