Effect of deoxycytidine on frequency of sister chromatid exchanges in human lymphocytes detected with 5-bromodeoxyuridine
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The frequency of sister chromatid exchanges (SCE) during abolition of the inhibitory action of 5-bromodeoxyuridine (BdU, 0.05 mM) on DNA synthesis by means of deoxycytidine (dC, 0.1 and 0.01 mM) was investigated in cultures of lymphocytes from the blood of three normal individuals. The frequency of SCE was significantly increased in all three experiments in the presence of dC in a concentration of 0.1 mM. Parallel with the increase in the frequency of SCE, dC also increased the yield of second-division metaphases. Elevation of the SCE level under the influence of dC was connected with its normalizing action on DNA replication.
Key Wordssister chromatid exchanges 5-bromodeoxyuridine deoxycytidine
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