Abstract
Stromal fibroblasts from monolayer cultures of thymus, spleen, and bone marrow cells, when added to nonadherent suspension cultures of spleen cells have a significant influence on the accumulation of antibody-forming cells (AFC). Stromal fibroblasts of thymic origin stimulate-AFC formation, whereas those of bone-marrow origin inhibit antibody formation in these cultures. Stromal fibroblasts of splenic origin have the same effect on AFC formation as adherent cells. Stromal fibroblasts irradiated in a dose of 5000 R have the same action as unirradiated cells. The action of stromal fibroblasts on antibody formation in culture is manifested only if they adhere to the surface of the culture vessel.
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Kulagina, N.N., Sidorenko, A.V. Effect of stromal fibroblasts on antibody formation in nonadherent spleen cultures. Bull Exp Biol Med 85, 764–766 (1978). https://doi.org/10.1007/BF00806159
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DOI: https://doi.org/10.1007/BF00806159