Amino Acids

, Volume 2, Issue 1–2, pp 103–109 | Cite as

Separation and enantiomer determination ofOPA-derivatised amino acids by using capillary zone electrophoresis

  • L. Kang
  • R. H. Buck


Amino acids react with OPA and chiral mercaptans to give diastereomeric isoindole derivatives. The resolution of these diastereomers was investigated by micellar electrokinetic chromatography (MECC) and free solution capillary electrophoresis. MECC with SDS as micellar phase allows to separate the amino acid derivatives and to resolve the diastereomers. The separation is influenced by the amount of detergent and the organic modifier added. Capillary zone electrophoresis offers a valuable alternative to the traditional methods for amino acid analysis and enantiomer determination.


Amino acids Capillary zone electrophresis Micellar electrokinetic chromatography OPA-derivatives Enantiomer determination 


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  1. 1.
    Abdalla S, Bayer E, Frank H (1987) Chromatographia 23: 83Google Scholar
  2. 2.
    Buck RH, Krummen K (1984) J Chromatogr 315: 279Google Scholar
  3. 3.
    Buck RH, Krummen K (1987) J Chromatogr 387: 255Google Scholar
  4. 4.
    Brückner H, Wittner H, Godel H (1989) J Chromatogr 476: 73Google Scholar
  5. 5.
    Euerby MR, Patridge LZ, Gibbons WA (1989) J Chromatogr 483: 239Google Scholar
  6. 6.
    Einarsson S, Josefsson B, Möller P, Sanchez D (1987) Anal Chem 59: 1191Google Scholar
  7. 7.
    Gassmann E, Kuo JE, Zare RN (1985) Science 230: 813Google Scholar
  8. 8.
    Gozel P, Gassmann E, Michelson H, Zare RN (1987) Anal Chem 59: 44Google Scholar
  9. 9.
    Liu J, Cobb KA, Novotny M (1988) J Chromatogr 468: 55Google Scholar
  10. 10.
    Tran D An, Blanc T (1990) J Chromatogr 516: 241Google Scholar
  11. 11.
    Terabe S, Shibata M, Miyashita Y (1989) J Chromatogr 480: 403Google Scholar
  12. 12.
    García Alvarez Coque MC, Medina Hernández MJ, Villanueva Camañas RM, Mongay Fernández C (1989) Anal Biochem 178: 1Google Scholar
  13. 13.
    Terabe S, Ozaki H, Otsuka K, Ichikawa K, Tsuchiya A, Ando A (1984) Anal Chem 56: 111Google Scholar
  14. 14.
    Nishi H, Fukuyama T, Matsuo M, Terabe S (1990) J Chromatogr 515: 233Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • L. Kang
    • 1
  • R. H. Buck
    • 1
  1. 1.Analytical Research and DevelopmentSandoz Pharma LtdBasleSwitzerland

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