Summary
Effects of arginine deficiency and hyperammonemia on the brain concentrations of amino acids and urea cycle enzyme activities in young and adult ferrets were investigated. Only young ferrets developed hyperammonemia and encephalopathy immediately after consuming the arginine-free diet. Brain ornithine and citrulline concentrations in young ferrets fed arginine containing diet were significantly lower than those in adult ferrets. Compared to rats and other animals, young and adult ferrets had lower concentrations of brain glutamic acid and glutamine. Unlike in other species, brain glutamine was not elevated in young, hyperammonemic ferrets. Brain arginase and glutamate dehydrogenase activities were significantly increased in young ferrets fed arginine-free diet. Young ferrets provide a useful animal model for investigating the neurotoxicity of acute hyperammonemia.
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Abbreviations
- ACD:
-
Arginine-containing diet
- AFD:
-
Arginine-free diet
References
Benjamin AM (1983) Ammonia metabolic interactions between neurons and glia. In: Glutamine, glutamate and GABA in central nervous system. Alan R Liss, New York, pp 399–414
Burns RA, Milner JA, Corbin JE (1981) Arginine: an indispensable amino acid for dogs. J Nutr 111: 1020–1024
Cooper AJL, Mora SN, Cruz NF, Gelbard AS (1985) Cerebral ammonia metabolism in hyperammonemic rats. J Neurochem 44: 1716–1723
Czarnecki GL, Baker DH (1984) Urea cycle function in the dog with emphasis on the role of arginine. J Nutr 114: 581–590
Deshmukh DR, Rusk CD (1989) Effects of arginine-free diet on urea cycle enzymes in young and adult ferrets. Enzyme 41: 168–174
Deshmukh DR, Maassab HF, Mason M (1982) Interactions of aspirin and other potential etiologic factors in an animal model of Reye's syndrome. Proc Natl Acad Sci (U.S.A.) 79: 7557–7560
Deshmukh DR, Mukhopadhyay A, Sarnaik AP, Portoles M (1991) Effect of arginine-free diet on plasma and tissue amino acids in young and adult ferrets. J Nutr Biochem 2: 72–78
Hawkins RA, Jessy J (1991) Hyperammonemia depresses glucose consumption throughout the brain. Biochem J 277: 697–703
Jesse J, Mans AM, DeJoseph MR, Hawkins RA (1990) Hyperammonaemia causes many of the changes found after postcaval shunt. Biochem J 272: 311–317
Jones ME, Anderson AD, Anderson C, Hodes C (1961) Citrulline synthesis in rat tissues. Arch Biochem Biophys 95: 499–507
Kamata S, Okada A, Watanabe T, Kawashima Y, Wada HJ (1980) Effects of dietary amino acids and transmitter amines in rats with a portacaval shunt. J Neurochem 35: 1190–1199
Lowry OJ, Rosenbrough NJ, Farr A, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275
Mondzack A, Ehrlich GE, Sigmiller JE (1965) An enzymatic determination of ammonia in biological fluids. J Lab Clin Med 66: 526–531
Morris JG, Rogers QR (1978) Arginine: An essential amino acid for cat. J Nutr 108: 1944–1953
Norenberg MD (1977) A light and electron microscopic study of experimental portalsystemic (Ammonia) encephalopathy. Lab Invest 36: 618–627
Nuzum CT, Snodgrass PJ (1975) Multiple assays of the five urea cycle enzymes in human liver homogenates. In: The urea cycle. John Wiley, New York, pp 325–349
Peraino C, Pitot HC (1963) Ornithine transaminase. Biochem Biophys Acta 73: 222–231
Portoles M, Minana M, Jorda A, Grisolia S (1985) Caffeine induced changes in the composition of the free amino acid pool of the cerebral cortex. Neurochem Res 10: 887–895
Rogers QR, Phang JM (1985) Deficiency of pyrroline-5-carboxylate synthase in the intestinal mucosa of the cat. J Nutr 115: 146–150
Sadasivudu B, Indira HR (1976) Studies on functional and metabolic role of urea cycle intermediates in brain. J Neurochem 27: 785–794
Schmidt E (1974) Glutamate dehydrogenase. In: Methods of enzymic analysis. Academic Press, New York, pp 650–659
Semon BA, Leung PMB, Rogers QR, Gietzen DW (1989) Plasma ammonia, plasma, brain and liver amino acids and urea cycle enzyme activities in rats fed ammonium acetate. J Nutr 119: 166–174
Stewart PM, Batshaw M, Valle D, Walser M (1981) Effects of arginine-free meals on ureagenesis in cats. Am J Physiol 241: E310–E315
Takahashi HT, Koehler RC, Brusilow SW, Traystman RJ (1991) Inhibition of brain glutamine accumulation prevents cerebral edema in hyperammonemic rats. Am J Physiol 261: H825-H829
Tarrab R, Rodriguez J, Haitron C, Palacois R, Soberon G (1974) Molecular forms of rat liver arginase. Isolation and characterization. Eur J Biochem 49: 457–468
Thomas PE, Deshmukh DR (1986) Effect of arginine-free diet on ammonia metabolism in young and adult ferrets. J Nutr 116: 545–551
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This work was presented, in part, at the annual meeting of the Midwest Society for Pediatric Research, Chicago, IL, 1991.
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Deshmukh, D.R., Mukhopadhyay, A., Sarnaik, A.P. et al. Effect of hyperammonemia on brain amino acids in young and adult ferrets. Amino Acids 5, 289–297 (1993). https://doi.org/10.1007/BF00805991
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DOI: https://doi.org/10.1007/BF00805991