Summary
The effect of (aminooxy)acetate, an inhibitor of aminotransferases, on the sulfate formation froml-cysteine andl-cysteinesulfinate in rat liver mitochondria was studied. Incubation of 10 mMl-cysteine with rat liver mitochondria at 37°C in the presence of 10 mM 2-oxoglutarate and 10 mM glutathione resulted in the formation of 4.60 and 1.52µmol of sulfate and thiosulfate, respectively, per 60 min per mitochondria obtained from 1 g of liver. Under the same conditions sulfate formation froml-cysteinesulfinate was 24.96µmol, but thiosulfate was not formed. The addition of (aminooxy)acetate at 2 mM or more completely inhibited the sulfate and thiosulfate formation froml-cysteine and the sulfate formation froml-cysteinesulfinate. These findings support our previous conclusion that cysteine transamination and 3-mercaptopyruvate pathway (MP pathway) are involved in the sulfate formation froml-cysteine in rat liver mitochondria (Ubuka et al., 1992).
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References
Griffith OW (1987) Mammalian sulfur amino acid metabolism: an overview. Methods Enzymol 143: 366–376
Hopper S, Segal HL (1962) Kinetic studies of rat liver glutamic-alanine transaminase. J Biol Chem 237: 3189–3195
Hopper S, Segal HL (1964) Comparative properties of glutamic-alanine transaminase from several sources. Arch Biochem Biophys 105: 501–505
Singer TP (1975) Oxidative metabolism of cysteine and cystine in animal tissues. In: Greenberg DM (ed) Metabolic pathways, 3rd edn, vol 7. Academic Press, New York, pp 535–546
Ubuka T, Umemura S, Yuasa S, Kinuta M, Watanabe K (1978) Purification and characterization of mitochondrial cysteine aminotransferase from rat liver. Physiol Chem Phys 10: 483–500
Ubuka T, Kinuta M, Akagi R, Kiguchi S, Azumi M (1982) Reaction of S-(2-amino-2-carboxyethylsulfonyl)-l-cysteine with sulfite: synthesis of S-sulfocysteine andl-alanine 3-sulfinic acid and application to the determination of sulfite. Anal Biochem 126: 273–277
Ubuka T, Yuasa S, Ohta J, Masuoka N, Yao K, Kinuta M (1990) Formation of sulfate froml-cysteine in rat liver mitochondria. Acta Med Okayama 44: 55–64
Ubuka T, Ohta J, Yao W-B, Abe T, Teraoka T, Kurozumi Y (1992)l-Cysteine metabolism via 3-mercaptopyruvate pathway and sulfate formation in rat liver mitochondria. Amino Acids 2: 143–155
Wainer A (1964) The production of sulfate from cysteine without the formation of free cysteinesulfinic acid. Biochem Biophys Res Commun 16: 141–144
Wainer A (1967) Mitochondrial oxidation of cysteine. Biochim Biophys Acta 141: 466–472
Wallach DP (1961) Studies on the GABA pathway — I The inhibition ofγ-amino butyric acid-α-ketoglutaric acid transaminase in vitro and in vivo by U-7524 (amino-oxyacetic acid). Biochem Pharmacol 5: 323–331
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Teraoka, T., Ohta, J., Abe, T. et al. Inhibition of sulfate-forming activity in rat liver mitochondria by (aminooxy)acetate. Amino Acids 5, 245–251 (1993). https://doi.org/10.1007/BF00805987
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DOI: https://doi.org/10.1007/BF00805987