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Melanotropin-potentiating factor isolated from filtrate of uremic patients suffering from melanosis and carbohydrate intolerance

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Summary

Following the characterization of a hexadecapeptide,α-endorphin, melanotropin-potentiating factor (MPF) was isolated from the filtrate of uremic patients suffering from melanosis and carbohydrate intolerance. The structure of MPF has been determined on the bases of chemical and physicochemical examinations which included HPLC, Edman sequence analysis combined analysis of the amino acid composition and FAB-MS analysis. An accumulation of MPF might be a cause of melanosis in uremic patients.

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Abbreviations

Et3N:

triethylamine

TFA:

trifluoroacetic acid

HF:

hydrogen fluoride

CH3CN:

acetonitrile

DCC:

dicyclohexyl-carbidimide

HOBT:

N-hydroxybenzotriazole

OBzl:

benzyl ester

Boc:

tert-butoxycarbonyl

Z:

bebzyloxycarbonyl

EtOH:

ethanol

ECUM:

extracorporeal ultrafiltration method

FAB-MS:

fast atom bombardment spectrometry

HPLC:

high-performance liquid chromatography

TLC:

thin-layer chromatography

AcOH:

acetic acid

AP-M:

aminopeptidase-M

PTH:

phenylthiohydantoin

References

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Authors and Affiliations

  1. Kidney Research Laboratory, Kojinkai, 1-6 Tsutsujigaoka 2-chome, Miyagino-ku, 980, Sendai, Japan

    T. Abiko & H. Sekino

Authors
  1. T. Abiko
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  2. H. Sekino
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Abiko, T., Sekino, H. Melanotropin-potentiating factor isolated from filtrate of uremic patients suffering from melanosis and carbohydrate intolerance. Amino Acids 9, 229–234 (1995). https://doi.org/10.1007/BF00805954

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  • DOI: https://doi.org/10.1007/BF00805954

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