Bulletin of Experimental Biology and Medicine

, Volume 84, Issue 3, pp 1368–1371 | Cite as

Improved histochemical method of demonstrating open capillaries

  • S. Doležel
  • S. M. Shenderov


A modification of Pickworth's method for demonstrating open capillaries is described. The distinguishing feature of this method is freezing the organ in situ with liquid propane, cooled with liquid nitrogen, followed by lyophilization of the specimen and fixation with gaseous formaldehyde. The sections are stained strictly in accordance with Pickworth's method.

Key Words

open capillaries their demonstration Pickworth's method modification 


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Copyright information

© Plenum Publishing Corporation 1978

Authors and Affiliations

  • S. Doležel
  • S. M. Shenderov

There are no affiliations available

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