Skip to main content
SpringerLink
Log in

Proliferation and cytotoxicity of thymus lymphocytes in vitro

  • Microbiology and Immunology
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

The proliferative and cytolytic activity of lymphocytes from the spleen and intact thymus was compared after alloimmunization. The number of living cells and of DNA-synthesizing cells in a monoculture of thymocytes was 90–97% less, and in a mixed culture of thymus cells about 80% less that the corresponding number of spleen cells. The index of stimulation of immune thymocytes was several times greater than that of immune spleen cells. The peak of cytotoxicity was observed on the fourth to fifth day of stimulation, when the cytolytic activity of the immune thymocytes was close to the activity of immune spleen cells. The low DNA synthesis and the considerable cytotoxic activity of the immune thymocytes mean that stimulation of thymus cells in vitro can be used to obtain a cell population with a high content of cytolytic T lymphocytes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. G. I. Drizlikh, A. V. Andreev, I. F. Kotomina, et al., Byull. Éksp. Biol. Med., No. 3, 340 (1976).

    Google Scholar 

  2. L. C. Andersson, Scand. J. Immunol.,2, 75 (1973).

    Google Scholar 

  3. W. Andersson and H. Blomgren, Cell Immunol.,1, 362 (1970).

    Google Scholar 

  4. B. Bennett, J. Immunol.,95, 656 (1965).

    Google Scholar 

  5. J. C. Cerottini, H. D. Engers, H. R. McDonald, et al., J. Exp. Med.,130, 703 (1974).

    Google Scholar 

  6. M. Nabholz, J. Vives, H. M. Young, et al., Eur. J. Immunol.,4, 378 (1974).

    Google Scholar 

  7. P. Perlmann and G. Holm, Adv. Immunol.,11, 117 (1969).

    Google Scholar 

  8. I. A. Ramshaw and C. R. Parish, Cell. Immunol.,21, 226 (1976).

    Google Scholar 

  9. R. M. Torer and C. S. Henney, Nature (London),262, 75 (1976).

    Google Scholar 

  10. H. Wagner, Immunology,109, 630 (1972).

    Google Scholar 

  11. H. Wagner, J. Exp. Med.,138, 1679 (1973).

    Google Scholar 

  12. H. Wagner, A. W. Harris, and M. Feldman, Cell. Immunol.,4, 39 (1972).

    Google Scholar 

Download references

Authors
  1. S. N. Bykovskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. V. Sergeev
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. M. Luchanskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. A. Ivanov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. O. Raushenbakh
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bykovskaya, S.N., Sergeev, A.V., Luchanskaya, L.M. et al. Proliferation and cytotoxicity of thymus lymphocytes in vitro. Bull Exp Biol Med 84, 1311–1314 (1977). https://doi.org/10.1007/BF00805893

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00805893

Key Words

Search

Navigation