Molecular Biology Reports

, Volume 7, Issue 4, pp 241–245 | Cite as

Lipophilic proteins of Friend erythroleukemia cells

  • M. Semmel
Article
  • 16 Downloads

Abstract

Lipophilic proteins can be extracted from Friend mouse erythroleukemia cells (MELC) with acidic chloroform-methanol. The acidic extract contains at least 4 polypeptides of apparent M. W. 5, 9.5, 14 and 17 kdaltons as determined by SDS-polyacrylamide gel electrophoresis (PAGE). Delipidation of the extract with ether causes the formation of polymers of an apparent molecular weight ranging from 25 to 85 kdaltons, and strong binding of aminoacids, sugars and phospholipids, in particular phosphatidylinositol and phosphatidylethanolamine, to the polypeptides. Though the majority of the lipophilic proteins are of cellular origin, part of the polypeptides of M.W. 14 and 17 kdaltons may be viral components.

Keywords

Polymer Ether Sugar Molecular Weight Polypeptide 

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References

  1. 1.
    MarksP. A. & RifkindR. A., 1978. Ann. Rev. Biochim 47: 419–448.Google Scholar
  2. 2.
    LymanG. H., PreislerH. D. & PapahajopoulosD., 1976. Nature 262: 360–363.Google Scholar
  3. 3.
    FolchJ. & LeesM., 1951. J1. Biol. Chem. 191: 807–817.Google Scholar
  4. 4.
    GregoriadesA., 1973. Virology 54: 369–383.Google Scholar
  5. 5.
    LeesM., SakuraJ. D., SapirsteinV. S. & CuratoloW., 1979. Biochim. Biophys. Acta 559: 209–230.Google Scholar
  6. 6.
    AudubertF. & SemmelM., 1979. Biochim. Biophys. Res. Comm. 91: 416–426.Google Scholar
  7. 7.
    YamaneI., MatsuyaY. & ImboK., 1968. Proc. Soc. Exp. Biol. 127: 335–336.Google Scholar
  8. 8.
    LaemmliU. K., 1970. Nature 227: 680–685.Google Scholar
  9. 9.
    BonnerM. W. & LaskeyR. A., 1974. Europ. J1. Biochem. 46: 83–88.Google Scholar
  10. 10.
    ClevelandD. W. & FischerS. G., 1977. J. Biol. Chem. 252: 1102–1106.Google Scholar
  11. 11.
    LowryO. H., RosebroughN. J., FarrA. L. & RandallR. J., 1951. J1. Biol. Chem. 193: 265–275.Google Scholar
  12. 12.
    ChanD. S. & LeesM. B., 1974. Biochemistry 13: 2704–2710.Google Scholar
  13. 13.
    Bartlett, G. R., 1959. J1. Biol. Chem. 466–468.Google Scholar
  14. 14.
    RouserA. KritchevskyG., YamamotoA., SittonG., GalliC. & BaumanA. J., 1969. Methods in Enzymology 14: 272–317, Ac. Press.Google Scholar
  15. 15.
    TsueiD., HaubenstockH., RevoltellaR., FriendC., 1979. Jl Virol. 31: 178–183.Google Scholar
  16. 16.
    Audubert, F. & Semmel, M. Biochim. Biophys. Res. Comm. in press.Google Scholar

Copyright information

© Dr W. Junk Publishers 1981

Authors and Affiliations

  • M. Semmel
    • 1
  1. 1.Groupe 8 du C.N.R.S.Institut Gustave-RoussyVillejuifFrance

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