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Molecular Biology Reports

, Volume 13, Issue 1, pp 53–58 | Cite as

Occurrence of 40 S·polysomal complexes in polysome profiles of reticulocyte lysates

  • Piet W. L. Tas
  • Oskar H. W. Martini
Article
  • 51 Downloads

Abstract

In most reticulocyte lysates 40 S · polysomal complexes have such a short lifetime that they will not show up in the polysome profile. Here we describe a reticulocyte lysate where these 40 S · polysomal complexes apparently have a highly increased lifetime and therefore these complexes can be seen in the polysome profile as shoulders on the di-, tri- and tetrasome peak. The presence of these complexes in lysates probably signals an increased speed in the association of 40 S subunits with mRNA since similar alterations as described above show up in the polysome profile of normal lysates to which native ribosomal 40 S subunits were added.

Key words

reticulocyte lysates 

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Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • Piet W. L. Tas
    • 1
  • Oskar H. W. Martini
    • 2
  1. 1.Institut für Virologie und Immunbiologie der Universität WürzburgWürzburgGermany
  2. 2.Abtlg. Molekularbiologie der Universität HamburgHamburg 13Germany

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