Possible nuclease activity of the T-antigen of virus SV-40
- 16 Downloads
A preparation of the T-antigen of virus SV-40 was isolated from an extract of golden hamster tumors by precipitation with ammonium sulfate followed by fractionation on DEAE-cellulose. Despite 100-fold purification of the preparation it contained traces of cell proteins as impurities. Treatment of calf thymus DNA with the preparation of T-antigen in the presence of magnesium ions considerably reduced the viscosity of the DNA solution during the first hour of incubation. The T-antigen, if inactivated by heating, or the analogous fraction from normal hamster tissues had no such action. During centirfugation in a sucrose gradient the sedimentation constant of hamster DNA was reduced after treatment with T-antigen from 28 S to 16 S, corresponding to a reduction of about 4–5 times in the molecular weight of the DNA. It can be concluded from these results that the partially purified preparation of T-antigen of virus SV-40 possesses endonuclease activity.
Key Wordsvirus carcinogenesis T-antigen of virus SV-40 nuclease activity
Unable to display preview. Download preview PDF.
- 1.Methods of Investigation of Nucleic Acids [in Russian], Moscow (1970).Google Scholar
- 2.V. S. Shapot, Nucleases [in Russian], Moscow (1968).Google Scholar
- 3.M. A. Shlyankevich, Uspekhi Sovr. Biol.,73, No. 2, 192 (1972).Google Scholar
- 4.S. Kit, J. L. Melnick, M. Anken, et al., J. Virol.,1, 684 (1967).Google Scholar
- 5.H. M. Lazarus, M. B. Sporn, J. M. Smith, et al., J. Virol.,1, 1093 (1967).Google Scholar