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Bulletin of Experimental Biology and Medicine

, Volume 79, Issue 1, pp 46–49 | Cite as

Possible nuclease activity of the T-antigen of virus SV-40

  • M. A. Shlyankevich
  • D. S. Pirtskhalaishvili
  • T. B. Prigozhina
  • O. B. Drize
  • V. S. Shapot
Immunology and Microbiology

Abstract

A preparation of the T-antigen of virus SV-40 was isolated from an extract of golden hamster tumors by precipitation with ammonium sulfate followed by fractionation on DEAE-cellulose. Despite 100-fold purification of the preparation it contained traces of cell proteins as impurities. Treatment of calf thymus DNA with the preparation of T-antigen in the presence of magnesium ions considerably reduced the viscosity of the DNA solution during the first hour of incubation. The T-antigen, if inactivated by heating, or the analogous fraction from normal hamster tissues had no such action. During centirfugation in a sucrose gradient the sedimentation constant of hamster DNA was reduced after treatment with T-antigen from 28 S to 16 S, corresponding to a reduction of about 4–5 times in the molecular weight of the DNA. It can be concluded from these results that the partially purified preparation of T-antigen of virus SV-40 possesses endonuclease activity.

Key Words

virus carcinogenesis T-antigen of virus SV-40 nuclease activity 

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Literature Cited

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    V. S. Shapot, Nucleases [in Russian], Moscow (1968).Google Scholar
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Copyright information

© Plenum Publishing Corporation 1975

Authors and Affiliations

  • M. A. Shlyankevich
    • 1
  • D. S. Pirtskhalaishvili
    • 1
  • T. B. Prigozhina
    • 1
  • O. B. Drize
    • 1
  • V. S. Shapot
    • 1
  1. 1.Academy of Medical Sciences of the USSRUSSR

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