Abstract
Methods of isolating NAD-glucohydrolase from the microsomal fraction of the heart were tested. NAD-glucohydrolase was found to pass into the soluble state under the influence of phospholipase A, Triton X-100, and Na cholate. NAD-glucohydrolase from heart muscle has unique properties: it is reversibly denatured by 6 M urea but is not inactivated by its own substrate (NAD) at pH 8.0; reversible denaturation by urea correlates for NAD-glucohydrolases isolated from other sources as a rule with inactivation of the enzyme in the presence of NAD at pH 8.0.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
G. M. Boriskina and L. A. Tseitlin, Dokl. Akad. Nauk SSSR,207, No. 2, 464 (1972).
A. Z. Prikhod'ko, Byull. Éksperim. Biol. i Med., No. 12, 23 (1973).
A. Z. Prikhod'ko, L. A. Tseitlin, and S. E. Severin, Biokhimiya,39, 123 (1974).
S. E. Severin, L. A. Tseitlin, and T. N. Druzhinina, Biokhimiya,28, 145 (1963).
S. G. Alivisatos and D. W. Woolley, J. Biol. Chem.,219, 823 (1956).
S. Green, Hoppe-Seylers Z. Physiol. Chem.353, 851 (1972).
S. Green and A. Dobrjansky, Biochemistry,10, 4533 (1971).
B. I. Davis, Ann. New York Acad. Sci.,121, 404 (1964).
O. H. Lowry, N. J. Rosebrough, A. L. Farr, et al., J. Biol. Chem.,193, 265 (1951).
N. O. Kaplan, Biochim. Biophys. Acta,56, 388 (1962).
N. Swislocki and N. O. Kaplan, J. Biol. Chem.,242, 1083 (1967).
Rights and permissions
About this article
Cite this article
Boriskina, G.M., Tseitlin, L.A. Solubilization and some properties of NAD-glucohydrolase isolated from rabbit heart. Bull Exp Biol Med 79, 33–35 (1975). https://doi.org/10.1007/BF00805498
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00805498