Abstract
A local form of the graph versus host reaction (GVHR) was induced in adult (CBA×C57BL)F1 hybrid mice by subcutaneous injection of semiallogeneic spleen, thymus, or bone marrow cells from CBA mice into the right hind footpad. The criteria of activity of the GVHR were an increase in the number of blast forms in the region of popliteal lymph node and in its weight 7 days after transplantation of cells. After transplantation of 5×106 and 20×106 spleen cells the absolute weight of the regional lymph node was increased by 3–5 times and was significantly higher than in the control (injection of living syngeneic or fragmented semiallogeneic cells from the same source). By contrast with the control, in the experimental animals the effect clearly depended directly on the dose of transplanted cells. Enlargement of the lymph nodes was accompanied by the regular appearance of blast forms in them. Thymus and bone marrow cells had a much weaker action than spleen cells.
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Mayanskii, D.N., Meilikhova, A.N. A study of the lymph-node type of graft versus host reaction in mice. Bull Exp Biol Med 81, 399–401 (1976). https://doi.org/10.1007/BF00804931
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DOI: https://doi.org/10.1007/BF00804931